Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400030, China.
Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China.
Int J Mol Sci. 2023 Dec 22;25(1):173. doi: 10.3390/ijms25010173.
ALKBH proteins, the homologs of AlkB dioxygenase, constitute a single-protein repair system that safeguards cellular DNA and RNA against the harmful effects of alkylating agents. ALKBH10B, the first discovered -methyladenosine (mA) demethylase in Arabidopsis (), has been shown to regulate plant growth, development, and stress responses. However, until now, the functional role of the plant ALKBH10B has solely been reported in arabidopsis, cotton, and poplar, leaving its functional implications in other plant species shrouded in mystery. In this study, we identified the AlkB homolog SlALKBH10B in tomato () through phylogenetic and gene expression analyses. exhibited a wide range of expression patterns and was induced by exogenous abscisic acid (ABA) and abiotic stresses. By employing CRISPR/Cas9 gene editing techniques to knock out , we observed an increased sensitivity of mutants to ABA treatment and upregulation of gene expression related to ABA synthesis and response. Furthermore, the mutants displayed an enhanced tolerance to drought and salt stress, characterized by higher water retention, accumulation of photosynthetic products, proline accumulation, and lower levels of reactive oxygen species and cellular damage. Collectively, these findings provide insights into the negative impact of on drought and salt tolerance in tomato plant, expanding our understanding of the biological functionality of .
ALKBH 蛋白是 AlkB 双加氧酶的同源物，构成了一个单一蛋白修复系统，可保护细胞 DNA 和 RNA 免受烷化剂的有害影响。拟南芥中首次发现的 -甲基腺苷（mA）去甲基酶 ALKBH10B（）已被证明可调节植物的生长、发育和应激反应。然而，到目前为止，植物 ALKBH10B 的功能作用仅在拟南芥、棉花和杨树中得到报道，其在其他植物物种中的功能意义仍不清楚。在这项研究中，我们通过系统发育和基因表达分析在番茄中鉴定出 AlkB 同源物 SlALKBH10B。SlALKBH10B 表现出广泛的表达模式，并受到外源脱落酸（ABA）和非生物胁迫的诱导。通过使用 CRISPR/Cas9 基因编辑技术敲除 SlALKBH10B，我们观察到突变体对 ABA 处理的敏感性增加，以及与 ABA 合成和响应相关的基因表达上调。此外，SlALKBH10B 突变体对干旱和盐胁迫表现出更高的耐受性，表现为更高的保水能力、光合产物积累、脯氨酸积累，以及较低的活性氧水平和细胞损伤。总的来说，这些发现深入了解了 SlALKBH10B 对番茄植物耐旱和耐盐性的负面影响，扩展了我们对 SlALKBH10B 生物学功能的理解。
