使用 UPLC-MS/MS 检测雄性和雌性小鼠吸入电子烟蒸气 4 周后尼古丁和可替宁的定量分析。

Nicotine and cotinine quantification after a 4-week inhalation of electronic cigarette vapors in male and female mice using UPLC-MS/MS.

机构信息

From the Department of Pharmacology and Toxicology (F. Alasmari, F. M. Alotaibi, A. F. Alasmari, Alqahtani, AL-Rejaie, Alshammari); from the Department of Pharmaceutics (Elzayat, Alanazi); from the Department of Pharmaceutical Chemistry (Attwa, Ahmad), College of Pharmacy, King Saud University, and from the Department of Forensic Sciences (M. M. Alotaibi, Abdelgadir), College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Kingdom of Saudi Arabia.

出版信息

Saudi Med J. 2022 Jul;43(7):678-686. doi: 10.15537/smj.2022.43.7.20220142.

OBJECTIVES

To detect the cotinine and nicotine serum concentrations of female and male C57BL/6J mice after a 4-week exposure to electronic (e)-cigarette vapors using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).

METHODS

This experimental study was carried out at an animal facility and laboratories, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia, between January and August 2020. A 4-week exposure to e-cigarettes was carried out using male and female mice and serum samples were obtained for cotinine and nicotine quantification using UPLC-MS/MS. The chromatographic procedures involved the use of a BEH HSS T3 C18 column (100 mm x 2.1 mm, 1.7 μm) with acetonitrile as a mobile phase and 0.1% formic acid (2:98 v/v).

RESULTS

The applied methodology has highly efficient properties of detection, estimation, and extraction, where the limit of quantification (LOQ) for nicotine was 0.57 ng/mL and limit of detection (LOD) for nicotine was 0.19 ng/mL, while the LOQ for cotinine was 1.11 ng/mL and LOD for cotinine was 0.38 ng/mL. The correlation coefficient was r>0.99 for both compounds. The average recovery rate was 101.6±1.33 for nicotine and 100.4±0.54 for cotinine, while the precision and accuracy for cotinine and nicotine were less than 6.1. The serum cotinine level was higher in males (433.7±19.55) than females (362.3±16.27).

CONCLUSION

This study showed that the gender factor might play a crucial role in nicotine metabolism.

目的

采用超高效液相色谱-串联质谱法（UPLC-MS/MS）检测 4 周电子（e）香烟蒸气暴露后雌性和雄性 C57BL/6J 小鼠的可替宁和尼古丁血清浓度。

方法

本实验研究于 2020 年 1 月至 8 月在沙特阿拉伯利雅得沙特国王大学药学院的动物设施和实验室进行。使用雄性和雌性小鼠进行为期 4 周的电子烟暴露，并用 UPLC-MS/MS 测定可替宁和尼古丁的血清样本。色谱程序涉及使用 BEH HSS T3 C18 柱（100mm x 2.1mm，1.7μm），以乙腈为流动相，0.1%甲酸（2：98v/v）。

结果

所应用的方法学具有高效的检测、估计和提取性能，尼古丁的定量限（LOQ）为 0.57ng/mL，检测限（LOD）为 0.19ng/mL，而可替宁的 LOQ 为 1.11ng/mL，LOD 为 0.38ng/mL。两种化合物的相关系数均大于 0.99。尼古丁的平均回收率为 101.6±1.33%，可替宁的平均回收率为 100.4±0.54%，而可替宁和尼古丁的精密度和准确度均小于 6.1。男性（433.7±19.55）的血清可替宁水平高于女性（362.3±16.27）。