Budowle B
Forensic Sci Int. 1987 Mar;33(3):187-96. doi: 10.1016/0379-0738(87)90127-7.
A method is described for subtyping group-specific component (Gc) derived from human bloodstains. Bloodstained cuttings were extracted in 6 M urea. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing in the pH 4.5-5.4 range. After isoelectric focusing, Gc was detected by immunofixation in cellulose acetate membranes. This method permitted the successful typing of Gc in at least four-month-old bloodstains maintained at room temperature. Bloodstains from 266 liquid blood samples of known origin were subjected to both this method and immunofixation conventional agarose gel electrophoresis with no phenotypic discrepancies observed. The Gc population data for Whites from Baltimore, Maryland, were homogeneous with white sample populations from other geographical locations within the U.S.A.; while Gc data from northern U.S.A. black sample populations appeared to be heterogeneous compared with a southern United States black sample population.
描述了一种对源自人类血迹的群体特异性成分（Gc）进行亚型分型的方法。将带血的切片在6M尿素中提取。提取物在pH 4.5 - 5.4范围内进行超薄层聚丙烯酰胺凝胶等电聚焦。等电聚焦后，在醋酸纤维素膜上通过免疫固定检测Gc。该方法能够成功地对室温下保存至少四个月的血迹中的Gc进行分型。对来自266份已知来源的液态血样的血迹同时采用此方法和免疫固定常规琼脂糖凝胶电泳，未观察到表型差异。来自马里兰州巴尔的摩的白人的Gc群体数据与美国其他地理位置的白人样本群体一致；而与美国南部黑人样本群体相比，美国北部黑人样本群体的Gc数据似乎具有异质性。
