Department of Chemistry, R & D Center for Membrane Technology at Chung Yuan Christian University, Chung Li, Taiwan 32023, Republic of China.
Department of Biomedical Engineering, Chung Yuan Christian University, Chung Li, Taiwan 32023, Republic of China.
ACS Appl Bio Mater. 2022 Aug 15;5(8):3778-3787. doi: 10.1021/acsabm.2c00323. Epub 2022 Jul 13.
Sulfonated copolyanilines (SPANs), SPAN-40 and SPAN-75, were prepared and applied in this tissue engineering study. SPAN scaffolds (SPANs) and control group polyaniline (PANI) were synthesized by performing oxidative polymerization. To further research the effects of neuron regeneration, PC12 cells were cultured on as-prepared PANI and SPANs with laminin (La) treatment under electrical stimulation. The effects on PC12 cell differentiation were investigated by controlling the amount of sulfonated groups (-SOH) in the SPAN chain, the electrical stimulation voltage, and the presence or absence of La coating. The adhesion and proliferation of cells increased with the degree of sulfonation; La and electrical stimulation further promoted neuronal cell differentiation as increased neurite length was demonstrated in the micrograph analyses. In summary, the sulfonated copolyaniline coated with La had the best effect on neuronal differentiation under electrical stimulation, suggesting its potential as a substrate for nerve tissue engineering.
磺化共聚苯胺（SPAN）、SPAN-40 和 SPAN-75 被制备并应用于这项组织工程研究中。SPAN 支架（SPANs）和对照组聚苯胺（PANI）通过氧化聚合合成。为了进一步研究神经元再生的影响，在电刺激下，将 PC12 细胞培养在预先制备的具有层粘连蛋白（La）处理的 PANI 和 SPANs 上。通过控制 SPAN 链中磺化基团（-SOH）的数量、电刺激电压以及是否存在 La 涂层来研究对 PC12 细胞分化的影响。细胞的黏附和增殖随着磺化程度的增加而增加；La 和电刺激进一步促进神经元细胞分化，通过显微分析证明了神经突长度的增加。综上所述，磺化共聚苯胺涂覆 La 在电刺激下对神经元分化的效果最好，表明其在神经组织工程中的潜在应用。
