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一种用于收集聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒的增强方法以及负载1,3,4,6-四乙酰基-2-叠氮基-2-脱氧-D-葡萄糖（Ac2AzGlc）的PLGA纳米颗粒的制备，用于高效和前瞻性的代谢处理。

An Augmented Method for Collecting PLGA Nanoparticles and the Fabrication of 1, 3, 4, 6-Tetra--acetyl-2-azido-2-deoxy-D-glucopyranose (Ac2AzGlc)-Loaded PLGA Nanoparticles for Efficient and Prospective Metabolic Processing.

作者信息

Parashar Shubham, Chauhan Charu, Rajasekharan Abhiraj, Rautela Jyoti, Jain Tanya, Raza Kaisar

机构信息

Department of Pharmacy, School of Chemical Sciences and Pharmacy, Central University of Rajasthan (CURAJ), Bandar Sindri, Ajmer, India.

Laboratory of Chemical Glycobiology, National Institute of Immunology (NII), New Delhi, India.

出版信息

Front Bioeng Biotechnol. 2022 Jun 27;10:833456. doi: 10.3389/fbioe.2022.833456. eCollection 2022.

DOI:10.3389/fbioe.2022.833456
PMID:35832404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9271679/
Abstract

We investigated two ways for fabricating 1, 3, 4, 6-tetra--acetyl-2-azido-2-deoxy-D-glucopyranose (Ac2AzGlc)-loaded poly (lactic-co-glycolic acid) PLGA nanoparticles in this article : 1) single emulsion solvent evaporation and 2) the nanoprecipitation method. Among the available methods of collecting nanoparticles using an ultra-high-speed centrifuge, we improvised a less-known method for collecting synthesized nanoparticles without a high-speed centrifuge, based on molecular weight (MW)-dependent centrifugal filters. These nanoparticles were collected in a tabletop centrifuge at a meager centrifugal force in the range of 200-300 xg whereas the conventional high-speed centrifuge method for nanoparticle recovery results in a hard nanoparticle pellet with poor resuspendability which hampers the yield and outcomes of the product. The Ac2AzGlc-loaded PLGA nanoparticles were spherical in shape with consistent and reliable nanometric particle size. The polydispersity indices were well within the acceptable limits. The preliminary studies in RAW 264.7 cell and C57BL/6 mice advocated efficient engineering in the former; however, the latter needs further confirmatory investigations. Preliminary studies with un-encapsulated Ac2AzGlc showed poor engineering of cardiac glycoproteins, opening up avenues for Ac2AzGlc-loaded nanoparticles for improved bioavailability and efficient metabolic engineering.

摘要

在本文中，我们研究了两种制备负载1,3,4,6-四乙酰基-2-叠氮基-2-脱氧-D-吡喃葡萄糖（Ac2AzGlc）的聚（乳酸-共-乙醇酸）（PLGA）纳米颗粒的方法：1）单乳液溶剂蒸发法和2）纳米沉淀法。在使用超高速离心机收集纳米颗粒的现有方法中，我们基于分子量（MW）依赖性离心过滤器，改进了一种无需高速离心机收集合成纳米颗粒的鲜为人知的方法。这些纳米颗粒在台式离心机中以200-300 xg范围内的微小离心力收集，而传统的高速离心机回收纳米颗粒的方法会导致纳米颗粒沉淀坚硬且再悬浮性差，这会影响产品的产量和结果。负载Ac2AzGlc的PLGA纳米颗粒呈球形，具有一致且可靠的纳米级粒径。多分散指数在可接受的范围内。在RAW 264.7细胞和C57BL/6小鼠中的初步研究表明前者具有高效的工程效果；然而，后者需要进一步的验证性研究。对未封装的Ac2AzGlc的初步研究表明心脏糖蛋白的工程效果不佳，这为负载Ac2AzGlc的纳米颗粒提高生物利用度和进行高效代谢工程开辟了道路。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f74b/9271679/7073459952a7/fbioe-10-833456-g010.jpg
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