Butler Catherine A, Adams Geoffrey G, Blum Jordan, Byrne Samantha J, Carpenter Lauren, Gussy Mark G, Calache Hanny, Catmull Deanne V, Reynolds Eric C, Dashper Stuart G
Centre for Oral Health Research, Melbourne Dental School, University of Melbourne, Carlton, Vic, Australia.
Child and Community Wellbeing Unit, Melbourne School of Population & Global Health, University of Melbourne, Carlton, Vic, Australia.
J Oral Microbiol. 2022 Jul 5;14(1):2096287. doi: 10.1080/20002297.2022.2096287. eCollection 2022.
Human microbiomes assemble in an ordered, reproducible manner yet there is limited information about early colonisation and development of bacterial communities that constitute the oral microbiome.
The aim of this study was to determine the effect of exposure to breastmilk on assembly of the infant oral microbiome during the first 20 months of life.
The oral microbiomes of 39 infants, 13 who were never breastfed and 26 who were breastfed for more than 10 months, from the longitudinal VicGeneration birth cohort study, were determined at four ages. In total, 519 bacterial taxa were identified and quantified in saliva by sequencing the V4 region of the bacterial genes.
There were significant differences in the development of the oral microbiomes of never breastfed and breastfed infants. Bacterial diversity was significantly higher in never breastfed infants at 2 months, due largely to an increased abundance of and species from the phylum compared with breastfed infants.
These differences likely reflect breastmilk playing a prebiotic role in selection of early-colonising, health-associated oral bacteria, such as the group. The microbiomes of both groups became more heterogenous following the introduction of solid foods.
人类微生物群以有序、可重复的方式组装，但关于构成口腔微生物群的细菌群落的早期定殖和发育的信息有限。
本研究的目的是确定在生命的前20个月接触母乳对婴儿口腔微生物群组装的影响。
在纵向的VicGeneration出生队列研究中，对39名婴儿的口腔微生物群进行了测定，其中13名从未接受母乳喂养，26名接受母乳喂养超过10个月，在四个年龄段进行了测定。通过对细菌基因的V4区域进行测序，总共在唾液中鉴定并定量了519个细菌分类群。
从未母乳喂养和母乳喂养婴儿的口腔微生物群发育存在显著差异。从未母乳喂养的婴儿在2个月时细菌多样性显著更高，这主要是由于与母乳喂养婴儿相比，拟杆菌门的物种丰度增加。
这些差异可能反映了母乳在选择早期定殖、与健康相关的口腔细菌（如双歧杆菌属）方面发挥益生元作用。在引入固体食物后，两组的微生物群变得更加多样化。
