Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Drosophila Research lab, Functional Foods and Nutraceuticals Unit, Biochemistry Department, Federal University of Technology, Akure, Nigeria.
Biomarkers. 2022 Nov;27(7):684-693. doi: 10.1080/1354750X.2022.2101693. Epub 2022 Jul 28.
We investigated the effect of dietary inclusions of Moringa seed (5% and 10%) on blood pressure, angiotensin-1 converting enzyme (ACE) activity, and gene expression, as well as redox status in hypertensive rats.
Wistar strain albino rats were fed moringa seed-based diets for two weeks prior L-NAME (40 mg/kg/day, ) administration for another ten days. Subsequently, the blood pressure was monitored. Furthermore, the kidney homogenates were assayed for ACE activity and gene expression, as well as oxidative stress markers.
The increased (systolic = 297 ± 59.30 mmHg; diastolic= 242 ± 51.96 mmHg) blood pressure, arginase activity, and reduced nitric oxide level were significantly ameliorated in hypertensive rats treated with the seed. However, the elevated ACE activity was significantly reduced but not the upregulated gene. Also, the reduced antioxidant enzyme activities were ameliorated with a significant downregulation in their regulator-Nrf2. Rutin (4.07 ± 0.02 mg/g) and quercitrin (4.06 ± 0.01 mg/g), among others, were found in the seed.
This study suggests that moringa seed offers its antihypertensive properties by acting as an ACE inhibitor but not its gene modulator, and also modulates the antioxidant system through interaction with Nrf2.
Moringa seed could act as an ACE inhibitor and not its gene modulator.
我们研究了饮食中添加辣木籽（5%和 10%）对高血压大鼠血压、血管紧张素转换酶（ACE）活性和基因表达以及氧化还原状态的影响。
Wistar 白化大鼠在给予 L-NAME（40mg/kg/天，）前两周喂食辣木籽饮食，然后再喂食十天。随后监测血压。此外，还测定肾匀浆中的 ACE 活性和基因表达以及氧化应激标志物。
用辣木籽处理的高血压大鼠的血压升高（收缩压=297±59.30mmHg；舒张压=242±51.96mmHg）、精氨酸酶活性和一氧化氮水平降低得到明显改善。然而，ACE 活性的升高显著降低，但基因的上调并未得到改善。此外，抗氧化酶活性降低，其调节因子 Nrf2 的表达下调。在种子中发现了芦丁（4.07±0.02mg/g）和槲皮素（4.06±0.01mg/g）等物质。
本研究表明，辣木籽通过作为 ACE 抑制剂而不是其基因调节剂发挥其降压作用，并且通过与 Nrf2 相互作用调节抗氧化系统。
辣木籽可以作为 ACE 抑制剂而不是其基因调节剂。
