Guangdong Provincial Key Laboratory of Nutrition and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Zhancui Food Co., Ltd, Chaozhou 515634, China.
Food Funct. 2022 Aug 1;13(15):8228-8242. doi: 10.1039/d2fo00747a.
This study is aimed to investigate the health-associated benefits of bergamot-dietary fibers (DFs) with a special emphasis on weight loss and lipid-lowering effects, as well as the potential mechanisms involved. The feeding experiment of Sprague-Dawley (SD) rats for 6 weeks showed that DFs had dose-dependent regulatory effects against metabolic syndrome and they controlled obesity by slowing down the rate of weight growth, and reduced body mass index (BMI) and Lee's index without affecting appetite. Furthermore, DFs inhibited increment in TG, TC, LDL-C levels and AI index caused by a high-fat diet, and improved the pathological abnormality of the liver. Western blot results showed that DFs significantly up-regulated the protein expression levels of LXRα and CYP7A1, and down-regulated the levels of SREBP-1c, FAS, ACC and SREBP-2 in the liver. QRT-PCR results showed that DFs up-regulated PGC-1α, PRDM16, UCP-1, and PPARγ in brown adipose tissue. These results suggest that DFs played an effective role in reducing weight and lipids levels by promoting the decomposition and transport of lipids in liver, increasing the energy consumption of brown adipose tissue. DFs intervention reduced the difference in the intestinal microflora between rats fed with a normal diet and those fed with a high-fat diet. Soluble dietary fiber (SDF) and total dietary fiber (TDF) showed better weight loss and hypolipidemic potential compared to insoluble dietary fiber (IDF) at the same dose. In conclusion, bergamot-derived DFs demonstrated the potential to lower blood cholesterol and body weight and could be used to develop novel functional foods for the prevention or treatment of obesity and hyperlipidemia.
本研究旨在探讨香柠檬膳食纤维（DFs）对健康的益处，尤其关注其减肥和降血脂作用，以及潜在的作用机制。SD 大鼠 6 周喂养实验表明，DFs 对代谢综合征具有剂量依赖性的调节作用，可通过减缓体重增长速度来控制肥胖，降低体重指数（BMI）和 Lee 指数，而不影响食欲。此外，DFs 抑制了高脂肪饮食引起的 TG、TC、LDL-C 水平和 AI 指数的增加，并改善了肝脏的病理异常。Western blot 结果表明，DFs 显著上调了 LXRα 和 CYP7A1 的蛋白表达水平，下调了肝脏中 SREBP-1c、FAS、ACC 和 SREBP-2 的水平。QRT-PCR 结果表明，DFs 上调了棕色脂肪组织中 PGC-1α、PRDM16、UCP-1 和 PPARγ 的表达。这些结果表明，DFs 通过促进肝脏中脂质的分解和转运，增加棕色脂肪组织的能量消耗，有效发挥减肥和降血脂作用。DFs 干预降低了正常饮食和高脂肪饮食喂养大鼠肠道微生物群的差异。与不溶性膳食纤维（IDF）相比，相同剂量的可溶性膳食纤维（SDF）和总膳食纤维（TDF）表现出更好的减肥和降血脂潜力。总之，香柠檬来源的 DFs 具有降低血液胆固醇和体重的潜力，可用于开发预防或治疗肥胖症和高血脂症的新型功能性食品。
