Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest-6, Romania.
Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
Anal Bioanal Chem. 2022 Sep;414(22):6521-6530. doi: 10.1007/s00216-022-04209-x. Epub 2022 Jul 14.
Arginine has an important role in the metabolomics of gastric cancer. Two 3D enantioselective needle stochastic sensors based on the physical immobilization of 2,3,7,8,12,13,17,18-octaethyl-21H,23H-porphine manganese(III) chloride (solution, 10 mol L) in graphene paste matrices decorated with N and S atoms were designed, characterized and validated for the enantioanalysis of arginine in whole blood and tissue samples. The signature values obtained for the enantiomers of arginine confirmed that the stochastic sensors are enantioselective. The lowest limit of quantification obtained for both enantiomers of arginine was 1 fmol L, while sensitivity of up to 10 s mol L was recorded for the stochastic sensors. High recoveries were obtained for the determination of one enantiomer in the presence of the other one; moreover, very good correlation was found between the results obtained with the two 3D enantioselective needle stochastic sensors.
精氨酸在胃癌的代谢组学中具有重要作用。基于物理固定化的 2,3,7,8,12,13,17,18-辛基-21H,23H-卟啉锰(III)氯化物（溶液，10mol L）在含有 N 和 S 原子的石墨烯糊剂基质中设计、表征和验证了两种 3D 对映选择性针式随机传感器，用于全血和组织样品中精氨酸的对映体分析。获得的精氨酸对映体特征值证实了随机传感器具有对映选择性。两种精氨酸对映体的最低定量限均为 1 fmol L，而随机传感器的灵敏度高达 10 s mol L。在存在一种对映体的情况下，对另一种对映体的测定获得了较高的回收率；此外，两种 3D 对映选择性针式随机传感器的结果之间发现了很好的相关性。
