Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
Food Chem. 2021 Aug 30;354:129570. doi: 10.1016/j.foodchem.2021.129570. Epub 2021 Mar 13.
While tissue fatty acid compositions reflect that of the dietary lipid source, little information is available on how dietary oils modify lipid class and molecular species profiles in hepatopancreas of crustacean. Herein, an 8-week nutritional trial and untargeted lipidomic analysis were used to investigate the impacts of dietary n-3 PUFA lipid sources including fish oil, krill oil and linseed oil on the lipidomic characteristics of hepatopancreas of swimming crab (Portunus trituberculatus). Dietary krill oil significantly increased distribution of 20:5n-3 and 22:6n-3 at sn-2 in phosphatidylcholine and phosphatidylethanolamine compared to fish oil. Fish oil intake promoted the deposition of 20:5n-3 and 22:6n-3 at sn-1,2,3 in triglyceride compared to linseed oil, which significantly increased the specific accumulation of 18:3n-3 at sn-1,3 in triglyceride and sn-2 in phosphatidylcholine and phosphatidylethanolamine. The study revealed metabolic responses to different dietary n-3 PUFA in swimming crab, which provided novel insight into the lipid nutrition of crustacean.
虽然组织中的脂肪酸组成反映了饮食中脂质来源的情况，但关于饮食中的油脂如何改变甲壳类动物肝胰腺中脂质类和分子种类的概况，目前相关信息还很有限。在此，我们采用为期 8 周的营养试验和非靶向脂质组学分析方法，研究了包括鱼油、磷虾油和亚麻籽油在内的 n-3PUFA 饮食脂质来源对三疣梭子蟹（Portunus trituberculatus）肝胰腺脂质组学特征的影响。与鱼油相比，饮食中的磷虾油显著增加了磷脂酰胆碱和磷脂酰乙醇胺中 sn-2 位置的 20:5n-3 和 22:6n-3 的分布。与亚麻籽油相比，鱼油摄入促进了甘油三酯中 sn-1、2、3 位置的 20:5n-3 和 22:6n-3 的沉积，这显著增加了甘油三酯中 sn-1、3 位置的 18:3n-3 和磷脂酰胆碱和磷脂酰乙醇胺中 sn-2 位置的特定积累。该研究揭示了不同 n-3PUFA 对三疣梭子蟹的代谢反应，为甲壳类动物的脂质营养提供了新的见解。
