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线粒体抑制复合物通过 ATG24 辅助的线粒体自噬来调节真菌的毒力。

Mitochondrial prohibitin complex regulates fungal virulence via ATG24-assisted mitophagy.

机构信息

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

出版信息

Commun Biol. 2022 Jul 14;5(1):698. doi: 10.1038/s42003-022-03666-5.

DOI:10.1038/s42003-022-03666-5
PMID:35835849
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9283515/
Abstract

Prohibitins are highly conserved eukaryotic proteins in mitochondria that function in various cellular processes. The roles of prohibitins in fungal virulence and their regulatory mechanisms are still unknown. Here, we identified the prohibitins ChPhb1 and ChPhb2 in a plant pathogenic fungus Colletotrichum higginsianum and investigated their roles in the virulence of this anthracnose fungus attacking crucifers. We demonstrate that ChPhb1 and ChPhb2 are required for the proper functioning of mitochondria, mitophagy and virulence. ChPhb1 and ChPhb2 interact with the autophagy-related protein ChATG24 in mitochondria, and ChATG24 shares similar functions with these proteins in mitophagy and virulence, suggesting that ChATG24 is involved in prohibitin-dependent mitophagy. ChPhb1 and ChPhb2 modulate the translocation of ChATG24 into mitochondria during mitophagy. The role of ChATG24 in mitophagy is further confirmed to be conserved in plant pathogenic fungi. Our study presents that prohibitins regulate fungal virulence by mediating ATG24-assisted mitophagy.

摘要

抑制素是高度保守的真核线粒体蛋白，在各种细胞过程中发挥作用。抑制素在真菌毒力中的作用及其调控机制尚不清楚。在这里，我们在植物病原菌炭疽菌中鉴定了抑制素 ChPhb1 和 ChPhb2，并研究了它们在攻击十字花科植物的炭疽菌毒力中的作用。我们证明 ChPhb1 和 ChPhb2 是线粒体、线粒体自噬和毒力正常发挥所必需的。ChPhb1 和 ChPhb2 与线粒体中的自噬相关蛋白 ChATG24 相互作用，ChATG24 在自噬和毒力方面与这些蛋白具有相似的功能，表明 ChATG24 参与了抑制素依赖性的线粒体自噬。ChPhb1 和 ChPhb2 调节 ChATG24 在自噬期间向线粒体的易位。ChATG24 在自噬中的作用在植物病原菌中进一步被证实是保守的。我们的研究表明，抑制素通过调节 ATG24 辅助的线粒体自噬来调节真菌毒力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/fc245a647c2b/42003_2022_3666_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/235456a93d41/42003_2022_3666_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/93f0610dbe0d/42003_2022_3666_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/0f3a68e7460c/42003_2022_3666_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/3d31467529a8/42003_2022_3666_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/a786931989a6/42003_2022_3666_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/f35c8784932d/42003_2022_3666_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/dcd571754776/42003_2022_3666_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/42b7f664ec45/42003_2022_3666_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/fc245a647c2b/42003_2022_3666_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/235456a93d41/42003_2022_3666_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/93f0610dbe0d/42003_2022_3666_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/0f3a68e7460c/42003_2022_3666_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/3d31467529a8/42003_2022_3666_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/a786931989a6/42003_2022_3666_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/f35c8784932d/42003_2022_3666_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/dcd571754776/42003_2022_3666_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/42b7f664ec45/42003_2022_3666_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0a/9283515/fc245a647c2b/42003_2022_3666_Fig9_HTML.jpg

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Mitochondrial fission is necessary for mitophagy, development and virulence of the insect pathogenic fungus Beauveria bassiana.
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