Department of Colorectal Cancer Surgery, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China.
Tianjin Marvel Medical Laboratory, Tianjin Marvelbio Technology Co., Ltd., Tianjin, 300381, China.
World J Surg Oncol. 2022 Jul 14;20(1):234. doi: 10.1186/s12957-022-02697-w.
Pyroptosis and related gasdermin family proteins play an important role in the tumorigenesis of colorectal cancer (CRC). However, the prognostic roles of pyroptosis-related genes (PRGs) and their relation to infiltrates of immune cells in the pathogenesis of CRC remain unclear. Using this study, we set up a prognostic gene pattern on the basis of 13 PRGs (AIM2, CASP1, CASP5, CASP6, CASP8, CASP9, ELANE, GPX4, GSDMD, NLRP7, NOD2, PJVK, and PRKACA) for CRC patients. A comprehensive bioinformatics analysis based on these genes was then performed. With the good AUC prediction value of the ROC curves, the group with high hazard first had a poorer survival prognosis than the group with low hazard. Second, we found that PRGs were significantly related to inflammation-associated genes and immune-associated genes in CRC. Then, we identified a correlation of PRGs with immune infiltrations in CRC. For instance, the abundances of resting NK cells resting and neutrophils were higher in the low hazard group than in the high hazard group. Overall, this work indicated that PRGs contributed to generate heterogeneity of the tumor microenvironment (TME) in CRC. This prognostic PRG model may provide a starting point for the early diagnosis and medication use of CRC.
细胞焦亡及相关的 Gasdermin 家族蛋白在结直肠癌（CRC）的发生发展中起重要作用。然而，细胞焦亡相关基因（PRGs）的预后作用及其与 CRC 发病机制中免疫细胞浸润的关系尚不清楚。本研究基于 13 个 PRGs（AIM2、CASP1、CASP5、CASP6、CASP8、CASP9、ELANE、GPX4、GSDMD、NLRP7、NOD2、PJVK 和 PRKACA）为 CRC 患者建立了一个预后基因模型。然后对这些基因进行了全面的生物信息学分析。基于这些基因的 ROC 曲线具有良好的 AUC 预测值，高风险组的患者首次生存预后较低风险组更差。其次，我们发现 PRGs 与 CRC 中炎症相关基因和免疫相关基因显著相关。然后，我们确定了 PRGs 与 CRC 中免疫浸润的相关性。例如，低风险组中静止 NK 细胞和中性粒细胞的丰度高于高风险组。总之，这项工作表明 PRGs 有助于产生 CRC 肿瘤微环境（TME）的异质性。该预后 PRG 模型可能为 CRC 的早期诊断和药物治疗提供一个起点。
