Lelieveld P, Aapro M S, van Lambalgen R, van den Berg K J
Invest New Drugs. 1986;4(4):367-71. doi: 10.1007/BF00173509.
Sodium azide (6 mg/ml) was used as a positive control for drug-induced lethality in an in vitro clonogenic assay. Petri dishes containing control and sodium azide treated cultures of WiDr cells were placed together in a large Petri dish and incubated at 37 degrees C in an atmosphere of 10% CO2 in air. No growth was observed. Control cells formed colonies only when the dishes were separated from the sodium azide dishes. Using a microtiter plate the toxic effect was inversely related to the distance of the test cultures from the sodium azide treated cultures. These results suggested the formation of a toxic gas or vapour from sodium azide under cell culture conditions, probably an azide. Chemical analysis was based on characteristic reactions, such as the production of a precipitate with silver ions or formation of a red-coloured complex with ferric salts. On a microtiter plate, a gradient of the expected precipitate or red colour was observed, the highest amounts adjacent to the wells containing sodium azide. These results show that sodium azide acts as a positive control of drug-induced lethality for in vitro clonogenic assays. However, the formation of a highly toxic vapour, most likely hydrazaic acid, makes it a less suitable standard.
叠氮化钠（6毫克/毫升）在体外克隆形成试验中用作药物诱导致死性的阳性对照。将含有WiDr细胞对照培养物和经叠氮化钠处理的培养物的培养皿一起放置在一个大培养皿中，并在37摄氏度、含10%二氧化碳的空气环境中孵育。未观察到生长。只有当培养皿与叠氮化钠培养皿分开时，对照细胞才形成菌落。使用微量滴定板时，毒性作用与测试培养物与经叠氮化钠处理的培养物之间的距离呈负相关。这些结果表明，在细胞培养条件下，叠氮化钠会形成一种有毒气体或蒸气，可能是一种叠氮化物。化学分析基于特征反应，例如与银离子产生沉淀或与铁盐形成红色络合物。在微量滴定板上，观察到预期沉淀或红色的梯度，最高量出现在含有叠氮化钠的孔附近。这些结果表明，叠氮化钠在体外克隆形成试验中可作为药物诱导致死性的阳性对照。然而，形成一种剧毒蒸气，很可能是氢叠氮酸，使其不太适合作为标准物质。
