Noguchi P, Wallace R, Johnson J, Earley E M, O'Brien S, Ferrone S, Pellegrino M A, Milstien J, Needy C, Browne W, Petricciani J
In Vitro. 1979 Jun;15(6):401-8. doi: 10.1007/BF02618407.
We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally, it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model cell line for tumor cell biology investigations.
我们描述了WiDr细胞系的建立及其特性，该细胞系源自人结肠癌。它在培养过程中产生癌胚抗原，倍增时间为15小时，接种效率为51%。WiDr细胞的HLA抗原谱和同工酶遗传特征（由八个基因-酶系统组成）与HeLa细胞不同。此外，WiDr细胞拥有三条标记染色体，同样与HeLa标记染色体不同。最后，它在四种不同的异种动物模型中具有高度致瘤性。基于这些研究，WiDr是肿瘤细胞生物学研究中一种有用的模型细胞系。
