School of Pharmaceutical Science, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan.
Second Clinical Medical College, Beijing University of Chinese Medicine, No. 11 North Third Ring East Road, Beijing 100029, China.
J Nutr Sci. 2022 Jul 11;11:e55. doi: 10.1017/jns.2022.43. eCollection 2022.
Excess body weight and hyperlipidaemia cause severe health problems and have social implications. Amycenone is an active substance extracted from Yamabushitake mushrooms with no reports of its activity against excess body weight and hyperlipidaemia. This research clarifies the effects and mechanisms of action of amycenone on the inhibition of body weight excess and hyperlipidaemia attenuation using KK- mice. Amycenone or water was administered to 8-week-old male KK- mice by gavage for 8 weeks. Their body weight and food intake were recorded during the experiment. At the end of the experimental period, the mice were dissected, and blood samples, lipid metabolism-related organs and tissues were collected and stored for further analysis. Amycenone treatment suppressed body weight gain and improved serum levels of fasting blood glucose and non-esterified fatty acids. Additionally, serum and hepatic cholesterol and triacylglycerol levels were reduced after this treatment, whereas the phosphorylation levels of AMPK, PKA and HSL increased and the expression level of FAS decreased. The protein level of C/EBPβ and gene expression level of were higher in the perirenal adipose tissue of amycenone-treated KK- mice. Furthermore, amycenone phosphorylated AMPK, PKA and ACC, and PPARγ expression was lower in the mesenteric adipose tissue. The phosphorylation levels of AMPK, LKB1, PKA and ACC were also induced, and FAS expression level was reduced in the liver of the amycenone-treated group. Amycenone could reduce excess body weight and attenuate hyperlipidaemia in KK- mice by inhibiting lipogenesis and promoting lipolysis through lipid metabolism pathway stimulation and fatty acid β-oxidation acceleration.
超重和高血脂会导致严重的健康问题，并具有社会影响。舞茸中提取的活性物质 Amycenone，目前尚未有其抑制体重增加和降低高血脂活性的报道。本研究使用 KK-小鼠阐明 Amycenone 抑制体重增加和降低高血脂的作用和机制。通过灌胃将 Amycenone 或水给予 8 周龄雄性 KK-小鼠 8 周。在实验过程中记录它们的体重和食物摄入量。在实验期末，对小鼠进行解剖，收集和储存血液样本、与脂质代谢相关的器官和组织，以备进一步分析。Amycenone 处理抑制了体重增加，改善了空腹血糖和非酯化脂肪酸的血清水平。此外，这种治疗后血清和肝胆固醇和三酰甘油水平降低，而 AMPK、PKA 和 HSL 的磷酸化水平增加，FAS 的表达水平降低。Amycenone 处理的 KK-小鼠肾周脂肪组织中 C/EBPβ 的蛋白水平和 的基因表达水平较高。此外，Amycenone 在肠系膜脂肪组织中使 PPARγ 的磷酸化 AMPK、PKA 和 ACC 的表达降低。AMPK、LKB1、PKA 和 ACC 的磷酸化水平也被诱导，并且 Amycenone 处理组的肝脏中 FAS 的表达水平降低。Amycenone 通过刺激脂质代谢途径和加速脂肪酸β氧化，抑制脂肪生成和促进脂肪分解，从而减少 KK-小鼠的超重和高血脂。
