Luo Jing, Zhu Xiaoqun, Liu Fei, Zhao Liang, Sun Zhenghao, Li Yan, Ye Lei, Li Weizu
Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University 218 Jixi, Hefei, Anhui, PR China.
Department of Neurology, The First Affiliated Hospital of Anhui Medical University Hefei, Anhui, PR China.
Am J Transl Res. 2022 Jun 15;14(6):4372-4379. eCollection 2022.
To examine the expression of serum miR-126 in patients with intracranial aneurysm and to explore its relationship with postoperative cerebral vasospasm.
In this retrospective study, 85 patients with intracranial aneurysms diagnosed and treated in our hospital were enrolled into the research group (RG), and 83 healthy volunteers who came to our hospital for physical examination were selected as the control group (CG). The serum miR-126 expression in both groups was examined by RT-PCR, and the relationship between the diagnostic value of miR-126 for intracranial aneurysm and postoperative cerebral vasospasm was analyzed. The serum inflammatory related factors in the patients were tested, and their correlation with miR-126 was assessed. The risk factors of postoperative cerebral vasospasm were evaluated by multiple factors.
The serum miR-126 expression in patients with intracranial aneurysm was obviously lower than that of participants in CG (P<0.05), and the AUC of miR-126 in diagnosing intracranial aneurysm was 0.945, which was of high diagnostic value. Serum inflammatory factors TNF-α and IL-6 were highly expressed in the serum of patients with intracranial aneurysm, which were positively correlated with the miR-126 level (P<0.05). After operation, the serum miR-126 level in patients with cerebral vasospasm was obviously higher than that of those without cerebral vasospasm, and the AUC of miR-126 for predicting cerebral vasospasm after operation was 0.859. Logistic regression analysis revealed that preoperative bleeding frequency, history of hypertension, Hunt-Hess grade and high expression of miR-126 were independent risk factors for cerebral vasospasm after operation in patients with intracranial aneurysm.
miR-126 is highly expressed in the serum of patients with intracranial aneurysm, so it may be used as a potential biomarker for the diagnosis of patients with intracranial aneurysm and the prediction of cerebral vasospasm after operation.
检测颅内动脉瘤患者血清miR-126的表达情况，并探讨其与术后脑血管痉挛的关系。
本回顾性研究中，将我院诊断并治疗的85例颅内动脉瘤患者纳入研究组（RG），选取来我院体检的83名健康志愿者作为对照组（CG）。采用RT-PCR检测两组血清miR-126表达，分析miR-126对颅内动脉瘤的诊断价值及与术后脑血管痉挛的关系。检测患者血清炎症相关因子，并评估其与miR-126的相关性。采用多因素评估术后脑血管痉挛的危险因素。
颅内动脉瘤患者血清miR-126表达明显低于CG组参与者（P<0.05），miR-126诊断颅内动脉瘤的AUC为0.945，具有较高诊断价值。颅内动脉瘤患者血清炎症因子TNF-α和IL-6高表达，与miR-126水平呈正相关（P<0.05）。术后，发生脑血管痉挛患者的血清miR-126水平明显高于未发生脑血管痉挛者，miR-126预测术后脑血管痉挛的AUC为0.859。Logistic回归分析显示，术前出血次数、高血压病史、Hunt-Hess分级及miR-126高表达是颅内动脉瘤患者术后脑血管痉挛的独立危险因素。
miR-126在颅内动脉瘤患者血清中高表达，可能作为诊断颅内动脉瘤患者及预测术后脑血管痉挛的潜在生物标志物。
