Reddy B Manohara, Anthony Johnson A M, Jagadeesh Kumar N, Venkatesh Boya, Jayamma N, Pandurangaiah Merum, Sudhakar Chinta
Plant Molecular Biology Laboratory, Department of Botany, Sri Krishnadevaraya University, Anantapur, India.
Department of Biotechnology, St. Josephs College (Autonomous), Bengaluru, India.
Front Plant Sci. 2022 Jun 28;13:868142. doi: 10.3389/fpls.2022.868142. eCollection 2022.
Cluster bean ( L.) is one of the multipurpose underexplored crops grown as green vegetable and for gum production in dryland areas. Cluster bean is known as relatively tolerant to drought and salinity stress. To elucidate the molecular mechanisms involved in the drought tolerance of cluster bean cultivar RGC-1025, RNA sequencing (RNA-seq) of the drought-stressed and control samples was performed. assembly of the reads resulted in 66,838 transcripts involving 203 pathways. Among these transcripts, differentially expressed gene (DEG) analysis resulted in some of the drought-responsive genes expressing 2, low temperature-induced 65 kDa protein (LDI65), putative vacuolar amino acid transporter, and late embryogenesis abundant protein (LEA 3). The analysis also reported drought-responsive transcription factors (TFs), such as NAC, WRKY, GRAS, and MYB families. The relative expression of genes by qRT-PCR revealed consistency with the DEG analysis. Key genes involved in the wax biosynthesis pathway were mapped using the DEG data analysis. These results were positively correlated with epicuticular wax content and the wax depositions on the leaf surfaces, as evidenced by scanning electron microscope (SEM) image analysis. Further, these findings support the fact that enhanced wax deposits on the leaf surface had played a crucial role in combating the drought stress in cluster beans under drought stress conditions. In addition, this study provided a set of unknown genes and TFs that could be a source of engineering tolerance against drought stress in cluster beans.
瓜尔豆（Cyamopsis tetragonoloba (L.) Taub.）是一种多用途但未得到充分研究的作物，在干旱地区作为绿色蔬菜种植并用于生产瓜尔豆胶。瓜尔豆被认为对干旱和盐胁迫具有较强的耐受性。为了阐明瓜尔豆品种RGC - 1025耐旱性所涉及的分子机制，对干旱胁迫和对照样本进行了RNA测序（RNA-seq）。读取序列的组装产生了66,838个转录本，涉及203条途径。在这些转录本中，差异表达基因（DEG）分析鉴定出了一些干旱响应基因，如低温诱导的65 kDa蛋白（LDI65）、假定的液泡氨基酸转运蛋白和晚期胚胎发生丰富蛋白（LEA 3）。分析还报告了干旱响应转录因子（TFs），如NAC、WRKY、GRAS和MYB家族。通过qRT-PCR对基因的相对表达分析显示与DEG分析结果一致。利用DEG数据分析绘制了蜡质生物合成途径中涉及的关键基因。扫描电子显微镜（SEM）图像分析表明，这些结果与表皮蜡质含量以及叶片表面的蜡质沉积呈正相关。此外，这些发现支持了在干旱胁迫条件下，叶片表面蜡质沉积增加在瓜尔豆抵御干旱胁迫中起关键作用这一事实。此外，本研究提供了一组未知基因和转录因子，它们可能是培育瓜尔豆耐旱性的工程基因来源。
