Department of Microbiology, Abdul Wali Khan University Mardan, Pakistan.
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Trop Biomed. 2022 Jun 1;39(2):185-190. doi: 10.47665/tb.39.2.006.
Plants are alternative source of natural medicines due to secondary active metabolites. Fagonia cretica extracts and Gradient High-Pressure Liquid Chromatography fractionations were checked against multidrug-resistant gastrointestinal pathogens including, Salmonella typhi, Escherichia coli and Shigella flexneri. ESI-MS/MS analysis of bioactive HPLC fractions was performed to elucidate antibacterial compounds. F. cretica extracts exhibited potential antibacterial activity. Twenty-four (24) HPLC fractions were obtained from methanol, ethanol and aqueous extracts of F. cretica. Eighteen (18) fractions showed antibacterial activity, while no activity was observed by the remaining six (6) fractions. HPLC fractions, F1 (25 ± 0.20 mm) and F2 (15 ± 0.12 mm) of aqueous extract exhibited activity against multidrug resistant GI pathogens. Gallic acid, quinic acid, cyclo-l-leu-l-pro, vidalenolone, liquirtigenin, rosmarinic acid and cerebronic acid were identified in F1 fraction of aqueous extract, while succinic acid, cyclo (l-Leul-Pro) and liquirtigenin were identified in F2 fraction of aqueous extract through ESI-MS/MS analysis. F. cretica extracts and HPLC fractions showed potential activity against MDR GI pathogens. Vidalenolone, Cyclo-1-leu-1-pro and Cerebronic acid are first time reported in F. cretica. Further characterization of bioactive compounds from F. cretica may be helpful to elucidate antibacterial therapeutic molecules.
由于次生代谢物的存在，植物成为了天然药物的替代来源。对包括伤寒沙门氏菌、大肠杆菌和福氏志贺菌在内的多种耐药胃肠道病原体进行了 Fagonia cretica 提取物和梯度高压液相色谱馏分的检测。对具有生物活性的 HPLC 馏分进行了 ESI-MS/MS 分析，以阐明抗菌化合物。F. cretica 提取物表现出潜在的抗菌活性。从 F. cretica 的甲醇、乙醇和水提取物中获得了二十四 (24) 个 HPLC 馏分。其中十八 (18) 个馏分具有抗菌活性，而其余六个 (6) 馏分则没有表现出活性。HPLC 馏分 F1（25±0.20mm）和 F2（15±0.12mm）对多重耐药性胃肠道病原体具有活性。在水提取物的 F1 馏分中鉴定出了没食子酸、奎宁酸、环-l-亮-l-脯氨酸、vidalenolone、liguiritigenin、迷迭香酸和cerebronic 酸，而在水提取物的 F2 馏分中鉴定出了琥珀酸、环（l-Leul-Pro）和 liguiritigenin 通过 ESI-MS/MS 分析。F. cretica 提取物和 HPLC 馏分显示出对 MDR GI 病原体的潜在活性。vidalenolone、Cyclo-1-leu-1-pro 和 Cerebronic 酸是首次在 F. cretica 中报道的。进一步对 F. cretica 中的生物活性化合物进行表征，可能有助于阐明抗菌治疗分子。
