Khan Ilyas, Rahman Hazir, Abd El-Salam Nasser M, Tawab Abdul, Hussain Anwar, Khan Taj Ali, Khan Usman Ali, Qasim Muhammad, Adnan Muhammad, Azizullah Azizullah, Murad Waheed, Jalal Abdullah, Muhammad Noor, Ullah Riaz
Departmen of Microbiology, Kohat University of Science & Technology, Kohat, Pakistan.
Departmen of Microbiology, Abdul Wali Khan University, Mardan, Pakistan.
BMC Complement Altern Med. 2017 May 3;17(1):247. doi: 10.1186/s12906-017-1766-4.
Medicinal plants are rich source of traditional herbal medicine around the globe. Most of the plant's therapeutic properties are due to the presence of secondary bioactive compounds.
The present study analyzed the High Pressure Liquid Chromatography (HPLC) fractions of Puncia granatum (peel) extracts (aqueous, chloroform, ethanol and hexane) against multidrug resistant bacterial pathogens (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus). All the fractions having antibacterial activity was processed for bioactive compounds identification using LC MS/MS analysis.
Among total HPLC fractions (n = 30), 4 HPLC fractions of P. granatum (peel) showed potential activity against MDR pathogens. Fraction 1 (F1) and fraction 4 (F4) collected from aqueous extract showed maximum activity against P. aeruginosa. Fraction 2 (F2) of hexane showed antibacterial activity against three pathogens, while ethanol F4 exhibited antibacterial activity against A. baumannii. The active fractions were processed for LC MS/MS analysis to identify bioactive compounds. Valoneic acid dilactone (aqueous F1 and F4), Hexoside (ethanol F4) and Coumaric acid (hexane F2) were identified as bioactive compounds in HPLC fractions.
Puncia granatum peel extracts HPLC fractions exhibited potential inhibitory activity against MDR bacterial human pathogens. Several bioactive compounds were identified from the HPLC fractions. Further characterization of these compounds may be helpful to conclude it as therapeutic lead molecules against MDR pathogens.
药用植物是全球传统草药的丰富来源。大多数植物的治疗特性归因于次生生物活性化合物的存在。
本研究分析了石榴（果皮）提取物（水提取物、氯仿提取物、乙醇提取物和己烷提取物）的高压液相色谱（HPLC）馏分对多重耐药细菌病原体（鲍曼不动杆菌、大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌）的作用。对所有具有抗菌活性的馏分进行处理，采用液相色谱-串联质谱（LC MS/MS）分析鉴定生物活性化合物。
在总共30个HPLC馏分中，石榴（果皮）的4个HPLC馏分对多重耐药病原体显示出潜在活性。从水提取物中收集的馏分1（F1）和馏分4（F4）对铜绿假单胞菌显示出最大活性。己烷馏分2（F2）对三种病原体具有抗菌活性，而乙醇馏分4对鲍曼不动杆菌具有抗菌活性。对活性馏分进行LC MS/MS分析以鉴定生物活性化合物。在HPLC馏分中鉴定出的生物活性化合物有戊酮酸双内酯（水提取物F1和F4）、己糖苷（乙醇提取物F4）和香豆酸（己烷提取物F2）。
石榴果皮提取物的HPLC馏分对多重耐药的人类细菌病原体表现出潜在的抑制活性。从HPLC馏分中鉴定出了几种生物活性化合物。对这些化合物的进一步表征可能有助于将其确定为针对多重耐药病原体的治疗先导分子。
