Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland.
Department of Restorative Dentistry, Institute of Science and Technology, Sao Paulo State University, Sao José dos Campos, São Paulo, Brazil.
Eur J Oral Sci. 2022 Oct;130(5):e12886. doi: 10.1111/eos.12886. Epub 2022 Jul 15.
Salivary pellicle was modified with bioproducts and we assessed the change in tooth color and the protection of enamel to erosion. Human enamel specimens were assigned to one of three solutions: grape seed extract or black tea (bioproducts), or deionized water (negative control); after which one half the specimens underwent erosive challenges. The specimens underwent 15 cycles involving salivary pellicle formation (10 min, 37°C), incubation in solution (2 min, 25°C), subsequent pellicle formation (90 min, 37°C). Half of the specimens was kept in a humid chamber and the other half was submitted to erosion (2 min, 1% citric acid). After 15 such cycles, the pellicle was removed. Tooth color and the surface reflection intensity were assessed after every five cycles and after pellicle removal. For non-eroded specimens, the exposure to bioproducts promoted significantly greater color change than the deionized water, with increases in yellow appearance. After pellicle removal, the color was similar in all non-eroded specimens. The bioproducts increased the surface reflection intensity over cycles. For the erosion-exposed specimens, erosion itself resulted in color change. Black tea and deionized water resulted in increased yellow appearance. Exposure to the bioproducts resulted in higher relative surface reflection intensity values over time, but only grape seed extract resulted in higher relative surface reflection intensity value at the time of pellicle removal. The bioproducts caused transient staining effect, which was reduced after pellicle removal. For enamel submitted to erosion, grape seed extract resulted in less color change and better protection of enamel against erosion than black tea or water.
唾液膜用生物制品进行了修饰，我们评估了牙齿颜色的变化和对脱矿的保护。人类牙釉质标本被分配到三种溶液中的一种：葡萄籽提取物或红茶（生物制品）或去离子水（阴性对照）；然后一半的标本进行了脱矿挑战。标本经过 15 个循环，涉及唾液膜的形成（10 分钟，37°C），在溶液中孵育（2 分钟，25°C），随后形成膜（90 分钟，37°C）。一半的标本保存在潮湿的室内，另一半暴露在脱矿液中（2 分钟，1%柠檬酸）。经过 15 个这样的循环后，去除了膜。每隔五个循环和去除膜后，评估膜的颜色和表面反射强度。对于未受侵蚀的标本，与去离子水相比，生物制品的暴露显著增加了颜色变化，使黄色外观增加。去除膜后，所有未受侵蚀的标本颜色相似。生物制品随着循环的增加而增加表面反射强度。对于暴露于脱矿液的标本，脱矿本身导致了颜色的变化。红茶和去离子水导致黄色外观增加。随着时间的推移，暴露于生物制品导致相对表面反射强度值增加，但只有在去除膜时，葡萄籽提取物才导致相对表面反射强度值增加。生物制品导致了短暂的染色效果，去除膜后效果降低。对于暴露于脱矿液的牙釉质，葡萄籽提取物导致的颜色变化较小，对牙釉质的脱矿保护优于红茶或水。
