Berggren-Thomas P L, Kaattari S, Hohenboken W D, Shrestha J N, Heaney D P
J Anim Sci. 1987 May;64(5):1302-12. doi: 10.2527/jas1987.6451302x.
Six hundred sixteen ewes of six strains were inoculated twice with ovalbumin in Freunds' incomplete adjuvant. To quantify the humoral immune response to the foreign antigen, blood samples were collected from all ewes 1 wk post-second injection. Blood samples were also collected between 4 and 40 h of age from their 709 lambs, to examine genetic differences in ability of lambs to acquire maternal anti-ovalbumin antibodies. Titers of anti-ovalbumin antibodies were determined using kinetic enzyme-linked immunosorbent assay (ELISA) techniques. Strain did not affect ewe immune response, but sire within strain was highly significant. In pregnant ewes, anti-ovalbumin antibody titers in 12- and 30-mo-old ewes were higher than those in 21-mo-old ewes. Number of lambs in utero did not significantly affect ewe immune response. Heritabilities of anti-ovalbumin titer from a paternal half-sib analysis were .27 +/- .17 for all ewes and .57 +/- .25 for only the pregnant ewes. The effect of strain of lamb on lamb anti-ovalbumin titer approached significance, and sire within strain was highly significant. Lamb anti-ovalbumin antibody concentration increased as time from birth to blood sampling increased to 18 h but declined thereafter. The size of the litter in which a lamb was born had a highly significant effect on the lamb's acquired immunity, with titer decreasing as litter size increased. The heritability estimate for lamb anti-ovalbumin antibody concentration from a paternal half-sib analysis was .38 +/- .11; it was .28 +/- .15 from the sire variance component of a full-sib analysis. When lamb titer was considered a maternal trait (lambs nested within their maternal grandsires within strains), the maternal grandsire variance component was negative. The average anti-ovalbumin antibody concentration of lambs that died between blood sample collection and 120 d of age was less than the average antibody concentration of lambs that survived (P less than .01).
将6个品系的616只母羊用弗氏不完全佐剂中的卵清蛋白接种两次。为了量化对外源抗原的体液免疫反应，在第二次注射后1周从所有母羊采集血样。还在其709只羔羊4至40小时龄之间采集血样，以检查羔羊获得母源抗卵清蛋白抗体能力的遗传差异。使用动力学酶联免疫吸附测定（ELISA）技术测定抗卵清蛋白抗体的滴度。品系不影响母羊的免疫反应，但品系内的父系具有高度显著性。在怀孕母羊中，12月龄和30月龄母羊的抗卵清蛋白抗体滴度高于21月龄母羊。子宫内羔羊的数量对母羊的免疫反应没有显著影响。通过父系半同胞分析得出，所有母羊抗卵清蛋白滴度的遗传力为0.27±0.17，仅怀孕母羊为0.57±0.25。羔羊品系对羔羊抗卵清蛋白滴度的影响接近显著性，品系内的父系具有高度显著性。羔羊抗卵清蛋白抗体浓度随着从出生到采血时间增加到18小时而增加，但此后下降。羔羊出生时的窝产仔数对羔羊获得性免疫有高度显著影响，滴度随窝产仔数增加而降低。通过父系半同胞分析得出的羔羊抗卵清蛋白抗体浓度的遗传力估计值为0.38±0.11；通过全同胞分析的父系方差分量得出的遗传力为0.28±0.15。当将羔羊滴度视为母体性状（品系内羔羊嵌套在其母系祖父内）时，母系祖父方差分量为负。在采血和120日龄之间死亡的羔羊的平均抗卵清蛋白抗体浓度低于存活羔羊的平均抗体浓度（P<0.01）。
