Reynolds G E, Griffin J F
Microbiology Department, University of Otago, Dunedin, New Zealand.
Vet Immunol Immunopathol. 1990 Jun;25(2):167-75. doi: 10.1016/0165-2427(90)90033-o.
Breeding ewes were immunised with clostridial vaccine using different inoculation schedules. Results, showing differences in the class of antibody produced, were heavily dependent on the vaccination regime used. Immunoglobulin M (IgM) levels were significantly lower in ewes given double doses of vaccine compared to ewes given a single inoculation or no treatment at all (P less than 0.01). Neonatal lambs showed significant de novo IgM production with interference in this antibody production in the lambs of ewes vaccinated with the clostridial vaccine (P less than 0.05). Immunoglobulin G1 (IgG1) levels were significantly increased in all lambs which had mothers vaccinated with the clostridial vaccine prior to or during pregnancy (P less than 0.025). The greatest quantity of IgG1 was transferred to lambs when their mothers were given a double injection with primary inoculation prior to conception and booster prepartum (P less than 0.025). No antigen specific immunoglobulin G2 (IgG2) was detected in the lambs. Ewes were also immunised with BSA and their isotype specific serum antibody response was compared with their respective lambs. There was no detectable anti-BSA IgM in the lambs of all groups of ewes though specific IgG1 antibodies could be readily detected in the lambs of hyperimmunised ewes. The efficiency of transfer was related directly to the ability of the adjuvant to maximise IgG1 production in the ewe. Although immunised ewes produced high levels of IgG2, this was not transferred passively to the lamb.
采用不同的接种方案，用梭菌疫苗对繁殖母羊进行免疫。结果表明，所产生抗体的类别存在差异，这在很大程度上取决于所采用的疫苗接种方案。与单次接种疫苗或未接受任何处理的母羊相比，接受双倍剂量疫苗的母羊体内免疫球蛋白M（IgM）水平显著降低（P<0.01）。新生羔羊表现出显著的IgM从头产生，而接种梭菌疫苗的母羊所产羔羊的这种抗体产生受到干扰（P<0.05）。所有在怀孕前或怀孕期间其母亲接种过梭菌疫苗的羔羊，其免疫球蛋白G1（IgG1）水平显著升高（P<0.025）。当母羊在配种前进行初次接种并在产前进行加强接种时，向羔羊转移的IgG1量最大（P<0.025）。在羔羊中未检测到抗原特异性免疫球蛋白G2（IgG2）。还用牛血清白蛋白（BSA）对母羊进行免疫，并将其同种型特异性血清抗体反应与其各自的羔羊进行比较。尽管在超免疫母羊所产羔羊中可以很容易地检测到特异性IgG1抗体，但在所有组母羊所产羔羊中均未检测到抗BSA IgM。转移效率直接与佐剂使母羊中IgG1产生最大化的能力相关。尽管免疫母羊产生了高水平的IgG2，但它并未被动转移至羔羊体内。
