《爪蟾外胚层细胞培养和成像方案》
Protocol for culturing and imaging of ectodermal cells from Xenopus.
机构信息
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1662, USA.
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1662, USA.
出版信息
STAR Protoc. 2022 Sep 16;3(3):101455. doi: 10.1016/j.xpro.2022.101455. Epub 2022 Jul 14.
The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustrate how this protocol can be applied to visualize lysosomes, macropinocytosis, focal adhesions, Wnt signaling, and cell migration. For complete details on the use and execution of this protocol, please refer to Tejeda-Muñoz et al. (2022).
爪蟾胚胎提供了一个有利的模型系统，可通过 DNA 或 mRNA 转染或反义技术敲低来轻易地移植基因。这里，我们提供了一个在组织培养中培养和成像离体爪蟾帽细胞的细胞生物学特性的方案。我们说明了如何应用该方案来可视化溶酶体、巨胞饮作用、焦点黏附、Wnt 信号通路和细胞迁移。如需详细了解该方案的使用和执行，请参考 Tejeda-Muñoz 等人（2022 年）。
Zotero 插件