Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
Department of Immunology, Danone Nutricia Research, Utrecht, the Netherlands; Laboratory of Translational Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, Netherlands.
Reprod Toxicol. 2022 Sep;112:109-118. doi: 10.1016/j.reprotox.2022.07.002. Epub 2022 Jul 14.
Deoxynivalenol (DON), a highly prevalent food contaminant, is known to induce reproductive and immunotoxicity in humans upon exposure. The present study focused on the consequences of exposure to DON during pregnancy for placental barrier and immune function, as well as fetal survival. Female mice received diets contaminated with DON (6.25 and 12.5 mg/kg of diet), starting immediately after mating until the end of the experiment. On day 17 of pregnancy the animals were killed, and maternal and fetal samples were collected for further analysis. Feeding on DON-contaminated diets decreased fetal survival, and DON was detected at significant levels in the fetus. Placentae from DON-exposed mice revealed a reduction in expression of junctional proteins, ZO-1, E-cadherin and claudins, upregulation of AHR mRNA expressions, and increase in IFN-ꝩ, IL-6 and IL-4 production. In conclusion, results of this study demonstrate harmful effects of DON on the course of pregnancy and fetal survival, which might be due to immunological changes in maternal immune organs and placenta. Altogether, these data underline the importance of the quality of maternal diet during pregnancy as they clearly demonstrate the potential harmful effects of a commonly present food-contaminant.
脱氧雪腐镰刀菌烯醇（DON）是一种普遍存在的食品污染物，已知人类在接触后会产生生殖和免疫毒性。本研究重点关注怀孕期间暴露于 DON 对胎盘屏障和免疫功能以及胎儿存活的影响。雌性小鼠在交配后立即开始接受含有 DON（饮食中 6.25 和 12.5mg/kg）的饮食，直至实验结束。在妊娠第 17 天，处死动物，并收集母体和胎儿样本进行进一步分析。用 DON 污染的饮食喂养会降低胎儿存活率，并且在胎儿中检测到显著水平的 DON。来自 DON 暴露的小鼠的胎盘显示出连接蛋白（ZO-1、E-钙粘蛋白和闭合蛋白）的表达减少，AHR mRNA 表达上调，IFN-ꝩ、IL-6 和 IL-4 的产生增加。总之，本研究的结果表明 DON 对妊娠过程和胎儿存活率有不良影响，这可能是由于母体免疫器官和胎盘的免疫变化所致。总的来说，这些数据强调了怀孕期间母体饮食质量的重要性，因为它们清楚地表明了一种常见的食品污染物的潜在有害影响。
