Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Animals, Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China.
Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, and Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute, Guangdong Ocean University, Shenzhen, 518120, China.
Sci Data. 2022 Jul 15;9(1):408. doi: 10.1038/s41597-022-01523-y.
Endemic to Australia, jade perch (Scortum barcoo) is a highly profitable freshwater bass species. It has extraordinarily high levels of omega-3 polyunsaturated fatty acids (PUFAs), which detailed genes involved in are largely unclear. Meanwhile, there were four chromosome-level bass species have been previous sequenced, while the bass ancestor genome karyotypes have not been estimated. Therefore, we sequenced, assembled and annotated a genome of jade perch to characterize the detailed genes for biosynthesis of omega-3 PUFAs and to deduce the bass ancestor genome karyotypes. We constructed a chromosome-level genome assembly with 24 pairs of chromosomes, 657.7 Mb in total length, and the contig and the scaffold N50 of 4.8 Mb and 28.6 Mb respectively. We also identified repetitive elements (accounting for 19.7% of the genome assembly) and predicted 26,905 protein-coding genes. Meanwhile, we performed genome-wide localization and characterization of several important genes encoding some key enzymes in the biosynthesis pathway of PUFAs. These genes may contribute to the high concentration of omega-3 in jade perch. Moreover, we conducted a series of comparative genomic analyses among four representative bass species at a chromosome level, resulting in a series of sequences of a deductive bass ancestor genome.
玉鲈原产于澳大利亚，是一种高利润的淡水鲈鱼物种。它含有极高水平的ω-3 多不饱和脂肪酸（PUFA），但其涉及的详细基因在很大程度上尚不清楚。同时，此前已经对四种染色体水平的鲈鱼物种进行了测序，而鲈鱼祖先的基因组染色体组型尚未被估计。因此，我们对玉鲈进行了测序、组装和注释，以表征其ω-3 PUFAs 生物合成的详细基因，并推导出鲈鱼祖先的基因组染色体组型。我们构建了一个染色体水平的基因组组装，共有 24 对染色体，总长 657.7Mb，其 contig 和 scaffold N50 分别为 4.8Mb 和 28.6Mb。我们还鉴定了重复元件（占基因组组装的 19.7%）和预测了 26905 个蛋白质编码基因。同时，我们对参与 PUFAs 生物合成途径的一些关键酶的几个重要基因进行了全基因组定位和特征分析。这些基因可能有助于玉鲈中ω-3 的高浓度。此外，我们在染色体水平上对四个代表性鲈鱼物种进行了一系列比较基因组分析，得到了一系列推导的鲈鱼祖先基因组序列。
