College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
CAS Key Laboratory of Experimental Marine Biology, CAS Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Sci Data. 2022 Aug 6;9(1):482. doi: 10.1038/s41597-022-01601-1.
The largemouth bass (Micropterus salmoides) has become a cosmopolitan species due to its widespread introduction as game or domesticated fish. Here a high-quality chromosome-level reference genome of M. salmoides was produced by combining Illumina paired-end sequencing, PacBio single molecule sequencing technique (SMRT) and High-through chromosome conformation capture (Hi-C) technologies. Ultimately, the genome was assembled into 844.88 Mb with a contig N50 of 15.68 Mb and scaffold N50 length of 35.77 Mb. About 99.9% assembly genome sequences (844.00 Mb) could be anchored to 23 chromosomes, and 98.03% assembly genome sequences could be ordered and directed. The genome contained 38.19% repeat sequences and 2693 noncoding RNAs. A total of 26,370 protein-coding genes from 3415 gene families were predicted, of which 97.69% were functionally annotated. The high-quality genome assembly will be a fundamental resource to study and understand how M. salmoides adapt to novel and changing environments around the world, and also be expected to contribute to the genetic breeding and other research.
大口黑鲈（Micropterus salmoides）由于其作为游戏或驯化鱼类的广泛引入，已成为一种世界性物种。在这里，我们通过结合 Illumina 配对末端测序、PacBio 单分子测序技术（SMRT）和高通量染色体构象捕获（Hi-C）技术，生成了大口黑鲈的高质量染色体水平参考基因组。最终，该基因组被组装成 844.88 Mb，具有 15.68 Mb 的 contig N50 和 35.77 Mb 的 scaffold N50 长度。约 99.9%的组装基因组序列（844.00 Mb）可以锚定到 23 条染色体上，98.03%的组装基因组序列可以进行排序和定向。该基因组包含 38.19%的重复序列和 2693 个非编码 RNA。总共预测了 26370 个来自 3415 个基因家族的蛋白质编码基因，其中 97.69%具有功能注释。高质量的基因组组装将成为研究和理解大口黑鲈如何适应世界各地新的和不断变化的环境的基础资源，也有望为遗传育种和其他研究做出贡献。
