Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
Ecotoxicol Environ Saf. 2022 Sep 1;242:113863. doi: 10.1016/j.ecoenv.2022.113863. Epub 2022 Jul 13.
We evaluated six plasma physiological indexes (epinephrine, cortisol, alkaline phosphatase, superoxide dismutase, reduced glutathione, and blood glucose) in turbot (Scophthalmus maximus) cultured in five ammonia concentrations (30.28, 35.90, 42.56, 50.47, and 59.82) at different exposure times (4, 8, 12, 24, and 48 h). Then, we conducted additive main effects and multiplicative interaction (AMMI) and genotype main effects and genotype × environment interaction (GGE) biplot analyses to analyze the genotype × ammonia interaction effects on the plasma physiological indexes. The AMMI analysis results revealed that the contributions of ammonia effect first decreased sharply (4-8 h), then decreased slowly (8-24 h), and then increased slowly (24-48 h). The contributions of genotype effect first decreased sharply (4-8 h), then increased slowly (8-24 h), and then decreased slowly (24-48 h). The contributions of genotype × ammonia interactions showed a sharp rise (4-8 h), a sharp decline (8-12 h), a slow decline (12-24 h), and finally a slow increase (24-48 h). The GGE biplot analysis revealed that alkaline phosphatase and reduced glutathione contents/activities are reliable biomarkers that can be used to establish an online early warning system for behavior numerical simulation.
我们评估了 5 种氨浓度（30.28、35.90、42.56、50.47 和 59.82）和 5 个暴露时间（4、8、12、24 和 48 h）下养殖大菱鲆（Scophthalmus maximus）的 6 种血浆生理指标（肾上腺素、皮质醇、碱性磷酸酶、超氧化物歧化酶、还原型谷胱甘肽和血糖）。然后，我们进行了加性主效应和乘法交互作用（AMMI）以及基因型主效应和基因型×环境互作（GGE）双标图分析，以分析基因型与氨互作对血浆生理指标的影响。AMMI 分析结果表明，氨效应的贡献先急剧下降（4-8 h），然后缓慢下降（8-24 h），然后缓慢上升（24-48 h）。基因型效应的贡献先急剧下降（4-8 h），然后缓慢上升（8-24 h），然后缓慢下降（24-48 h）。基因型×氨相互作用的贡献呈急剧上升（4-8 h）、急剧下降（8-12 h）、缓慢下降（12-24 h），最后缓慢上升（24-48 h）。GGE 双标图分析表明，碱性磷酸酶和还原型谷胱甘肽含量/活性是可靠的生物标志物，可用于建立行为数值模拟的在线预警系统。
