Wang Xinan, Ma Aijun, Huang Zhihui, Sun Zhibin, Liu Zhifeng
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Antioxidants (Basel). 2022 Oct 19;11(10):2062. doi: 10.3390/antiox11102062.
Three antioxidant properties (corresponding to the enzymes superoxide dismutase (SOD), catalase, and glutathione peroxidase) were measured in the livers of Scophthalmus maximus under different salinities/temperatures (5, 10, 20, 30, and 40‱/17, 20, 23, 25, and 28 °C). Split-plot (SP) analysis, additive main effects, and multiplicative interaction (AMMI) and genotype × environment interaction (GGE) biplots were used to analyze genotype × salinity/temperature interactions for antioxidant properties. The results of the SP analysis show that the activity of the three antioxidant properties was significantly (p < 0.01) affected by salinity/temperature, antioxidant, and salinity/temperature × antioxidant interaction. The results of the AMMI analysis for salinity reveal that the effect of genotype, salinity, and genotype × salinity interaction on antioxidant properties reached a significant level (p < 0.001); 92.1065%, 2.6256%, and 4.4360% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, salinity, and genotype × salinity interaction, respectively. The results of GGE biplot analysis for salinity reveal differences in the activity ranking of the three antioxidant properties under five salinities; this difference expands with an decrease or increase in salinity from 30‱ (optimum salinity). A salinity of 5‱ had the strongest ability to identify the three antioxidant properties. The five experimental salinities were divided into one region, and SOD activity was the highest in this region. In a comprehensive analysis of stability and activity, SOD had the best activity and stability. The results of AMMI analysis for temperature reveal that genotype, temperature, and genotype × temperature interaction had significant effects on the antioxidant properties (p < 0.001); 82.4720%, 4.0666%, and 12.0968% of the total sum of squares for antioxidant property activity were attributable to the effects of genotype, temperature, and genotype × temperature interaction, respectively. The results of GGE biplot analysis for temperature reveal a large difference in the activity ranking of antioxidant properties between 17 °C and the other four temperatures, while only small differences in the activity rankings were detected among the other four temperatures. The difference in the activity ranking of antioxidant properties was greatest between the temperatures of 17 and 20 °C. A temperature of 17 °C showed the strongest ability to distinguish the three antioxidant properties. Additionally, the five test temperatures were grouped into one region, and comprehensive analysis of activity and stability showed that SOD had the best activity and stability.
在不同盐度/温度条件（5、10、20、30和40‰/17、20、23、25和28℃）下，测定了大菱鲆肝脏中的三种抗氧化特性（分别对应超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶）。采用裂区（SP）分析、加性主效应和乘性互作（AMMI）以及基因型×环境互作（GGE）双标图来分析抗氧化特性的基因型×盐度/温度互作。SP分析结果表明，三种抗氧化特性的活性受到盐度/温度、抗氧化剂以及盐度/温度×抗氧化剂互作的显著影响（p < 0.01）。盐度的AMMI分析结果显示，基因型、盐度以及基因型×盐度互作对抗氧化特性的影响达到显著水平（p < 0.001）；抗氧化特性活性的总平方和中，分别有92.1065%、2.6256%和4.4360%可归因于基因型、盐度以及基因型×盐度互作的影响。盐度的GGE双标图分析结果显示，在五种盐度下三种抗氧化特性的活性排名存在差异；这种差异随着盐度从30‰（最适盐度）降低或升高而扩大。5‰的盐度识别三种抗氧化特性的能力最强。五个实验盐度被划分为一个区域，该区域内SOD活性最高。在稳定性和活性的综合分析中，SOD具有最佳的活性和稳定性。温度的AMMI分析结果显示，基因型、温度以及基因型×温度互作对抗氧化特性有显著影响（p < 0.001）；抗氧化特性活性的总平方和中，分别有82.4720%、4.0666%和12.0968%可归因于基因型、温度以及基因型×温度互作的影响。温度的GGE双标图分析结果显示，17℃与其他四个温度之间抗氧化特性的活性排名存在较大差异，而其他四个温度之间的活性排名差异较小。抗氧化特性活性排名的差异在17℃和20℃之间最大。17℃的温度区分三种抗氧化特性的能力最强。此外，五个测试温度被归为一个区域，活性和稳定性的综合分析表明，SOD具有最佳的活性和稳定性。
