Programa de Pós-graduação em Ciências Farmacêuticas (PCF), Universidade Estadual de Maringá (UEM), Av. Colombo 5790, 87.020-900 Maringá, Brazil.
Departamento de Farmácia, Universidade Estadual de Maringá (UEM), Av. Colombo 5790, 87.020-900 Maringá, Brazil.
J Biotechnol. 2022 Sep 10;356:8-18. doi: 10.1016/j.jbiotec.2022.07.001. Epub 2022 Jul 14.
The aim of this study was to establish a sustainable alternative callus culture of Cereus hildmannianus for the production and bioactive determination of phenolic compounds from this species. The conventional callus was cultivated using agar and Murashige and Skoog (MS) medium, while for the alternative culture the agar was replaced with a cotton support covered with filter paper and MS medium (incubated at 32 °C with photoperiod of 16 h), and the morphological characteristics and growth index were assessed (8 weeks). Extracts were obtained by maceration followed by partition, characterized by nuclear magnetic resonance - NMR and ultra-high performance liquid chromatography - UHPLC, quantified (phenolic compounds) by UV-Vis methods, and their antioxidant, antitumor activities, as well as cytotoxicity, were evaluated. The establishment of an alternative callus culture was carried out successfully. Characteristic signals of phenolic compounds were determined by NMR, and 46 compounds with fragment ions were identified using UHPLC analysis. The highest concentrations of phenolic compounds, and greatest antioxidant and antitumor activities, were obtained with the dichloromethane fractions of both callus tissue cultures, which were not cytotoxic. The callus culture from C. hildmannianus has shown promise as a source for the sustainable production of phenolic compounds with antioxidant and antiproliferative activities and thus, has potential use as a natural antitumor product.
本研究旨在建立一种可持续的仙人掌属 hildmannianus 愈伤组织培养方法，用于该物种中酚类化合物的生产和生物活性测定。常规愈伤组织使用琼脂和 Murashige 和 Skoog（MS）培养基进行培养，而对于替代培养，则用覆盖滤纸的棉花支架代替琼脂，并使用 MS 培养基（在 32°C 下孵育，光周期为 16 小时），评估其形态特征和生长指数（8 周）。通过浸提后进行分配提取提取物，通过核磁共振（NMR）和超高效液相色谱（UHPLC）进行表征，通过紫外可见分光光度法（UV-Vis）定量（酚类化合物），并评估其抗氧化、抗肿瘤活性以及细胞毒性。成功建立了替代愈伤组织培养。通过 NMR 确定了酚类化合物的特征信号，并通过 UHPLC 分析鉴定了 46 种具有碎片离子的化合物。在两种愈伤组织培养物的二氯甲烷部分中获得了最高浓度的酚类化合物、最强的抗氧化和抗肿瘤活性，且无细胞毒性。仙人掌属 hildmannianus 的愈伤组织培养显示出作为可持续生产具有抗氧化和抗增殖活性的酚类化合物的来源的潜力，因此具有作为天然抗肿瘤产品的潜在用途。
