Postle A D
J Chromatogr. 1987 Apr 10;415(2):241-51. doi: 10.1016/s0378-4347(00)83216-8.
Conditions are described for the efficient resolution by reversed-phase high-performance liquid chromatography on octadecylsilica stationary phases of individual molecular species of phosphatidylcholine on the basis of their fatty acyl composition. The effects are described of varying stationary phase carbon loading, mobile phase polarity and column temperature. Eluted peaks were detected by a sensitive post-column fluorescence system using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. This detection system permitted direct quantitation of phosphatidylcholine species irrespective of the degree of acyl unsaturation and eliminated the need for elaborate pre-column derivatization. The techniques were applied to the analysis of phosphatidylcholine isolated from rat liver and lung.
描述了基于其脂肪酰基组成，通过反相高效液相色谱法在十八烷基硅胶固定相上有效分离磷脂酰胆碱各个分子种类的条件。描述了改变固定相碳载量、流动相极性和柱温的影响。使用荧光探针1,6 - 二苯基 - 1,3,5 - 己三烯，通过灵敏的柱后荧光系统检测洗脱峰。该检测系统允许直接定量磷脂酰胆碱种类，而与酰基不饱和程度无关，并且无需复杂的柱前衍生化。这些技术应用于从大鼠肝脏和肺中分离的磷脂酰胆碱的分析。
