改进的高效液相色谱法用于测定从血浆中固相萃取后氯美噻唑的含量。

Improved high-performance liquid chromatographic procedure for the determination of chlormethiazole levels following solid-phase extraction from plasma.

作者信息

Hartley R, Lucock M, Becker M

出版信息

J Chromatogr. 1987 Apr 10;415(2):357-64. doi: 10.1016/s0378-4347(00)83227-2.

Abstract

An improved high-performance liquid chromatographic method for the determination of chlormethiazole levels in plasma is described. The drug is extracted from plasma using commercially available reversed-phase extraction columns; recovery values obtained using Sep-Pak C18 and Bond Elut C1, C2, C4, C6, C8, C18 columns are compared. Separation was achieved by reversed-phase chromatography, using a mobile phase consisting of 0.025 M sodium acetate buffer, pH 4.5-acetonitrile (67:33) at a flow-rate of 1.6 ml/min in conjunction with a 15-cm Jones Chromatography Apex ODS column. The analytical column was protected by a Waters Assoc. Guard-Pak module containing a Guard-Pak CN insert. Using ultraviolet detection at 254 nm chlormethiazole levels in the region of 50 ng/ml can be measured with only 500 microliter of plasma.

摘要

本文描述了一种改进的高效液相色谱法，用于测定血浆中氯美噻唑的含量。使用市售的反相萃取柱从血浆中提取药物；比较了使用Sep-Pak C18和Bond Elut C1、C2、C4、C6、C8、C18柱获得的回收率。通过反相色谱法进行分离，流动相由0.025 M醋酸钠缓冲液（pH 4.5）-乙腈（67:33）组成，流速为1.6 ml/min，结合15 cm的Jones Chromatography Apex ODS柱。分析柱由装有Guard-Pak CN插入物的Waters Assoc. Guard-Pak模块保护。使用254 nm的紫外检测，仅用500微升血浆就可以测量50 ng/ml左右的氯美噻唑含量。