Hartley R, Lucock M, Becker M
J Chromatogr. 1987 Apr 10;415(2):357-64. doi: 10.1016/s0378-4347(00)83227-2.
An improved high-performance liquid chromatographic method for the determination of chlormethiazole levels in plasma is described. The drug is extracted from plasma using commercially available reversed-phase extraction columns; recovery values obtained using Sep-Pak C18 and Bond Elut C1, C2, C4, C6, C8, C18 columns are compared. Separation was achieved by reversed-phase chromatography, using a mobile phase consisting of 0.025 M sodium acetate buffer, pH 4.5-acetonitrile (67:33) at a flow-rate of 1.6 ml/min in conjunction with a 15-cm Jones Chromatography Apex ODS column. The analytical column was protected by a Waters Assoc. Guard-Pak module containing a Guard-Pak CN insert. Using ultraviolet detection at 254 nm chlormethiazole levels in the region of 50 ng/ml can be measured with only 500 microliter of plasma.
本文描述了一种改进的高效液相色谱法，用于测定血浆中氯美噻唑的含量。使用市售的反相萃取柱从血浆中提取药物；比较了使用Sep-Pak C18和Bond Elut C1、C2、C4、C6、C8、C18柱获得的回收率。通过反相色谱法进行分离，流动相由0.025 M醋酸钠缓冲液（pH 4.5）-乙腈（67:33）组成，流速为1.6 ml/min，结合15 cm的Jones Chromatography Apex ODS柱。分析柱由装有Guard-Pak CN插入物的Waters Assoc. Guard-Pak模块保护。使用254 nm的紫外检测，仅用500微升血浆就可以测量50 ng/ml左右的氯美噻唑含量。
