Shibanoki S, Imamura Y, Arakawa Y, Ishikawa K
J Chromatogr. 1987 Apr 10;415(2):365-71. doi: 10.1016/s0378-4347(00)83228-4.
Dosulepin, 11-(3-dimethylaminopropylidene)-6,11-dihydrodibenzo [b,e]thiepin hydrochloride, is a thio analogue of amitriptyline and is used for the treatment of anxiety and affective disorders. The present study developed a simple and sensitive procedure for the determination of this compound and its metabolite, northiaden, by a combination of high-performance liquid chromatography with electrochemical detection. Hydrodynamic voltammograms demonstrated an optimal applied potential at 1300 mV for both dosulepin and northiaden. A mobile phase consisting of 0.1 M acetate buffer-acetonitrile-perchloric acid-trichloroacetic acid (50:50:2:1.5) provided the best separation of the drugs. The extraction procedure, which used a heptane-isoamyl alcohol (99:1) mixture, was successfully applied with a recovery of over 90%. A preliminary pharmacokinetic study was performed by the proposed method.
多塞平，即11-(3-二甲氨基亚丙基)-6,11-二氢二苯并[b,e]硫氮杂䓬盐酸盐，是阿米替林的硫代类似物，用于治疗焦虑症和情感障碍。本研究开发了一种简单灵敏的方法，通过高效液相色谱与电化学检测相结合来测定该化合物及其代谢产物去甲替林。流体动力伏安图表明，多塞平和去甲替林在1300 mV的外加电位下具有最佳效果。由0.1 M乙酸盐缓冲液-乙腈-高氯酸-三氯乙酸(50:50:2:1.5)组成的流动相能实现药物的最佳分离。使用庚烷-异戊醇(99:1)混合物的萃取方法成功应用，回收率超过90%。用所提出的方法进行了初步的药代动力学研究。
