Brodie R R, Chasseaud L F, Crampton E L, Hawkins D R, Risdall P C
J Int Med Res. 1977;5(6):387-90. doi: 10.1177/030006057300100201.
A method has been developed for the separation and measurement of dothiepin and the N-demethyl metabolites, northiaden, in human plasma or serum by high performance liquid chromatography. The method uses a structurally-related drug, amitriptyline, as an internal standard and provides a limit of detection of about 10 ng/ml for each component. At a concentration of 20 ng/ml, northiaden and dothiepin could be measured with +/-11% and +/- 6% of the mean respectively and at 200 ng/ml within +/-3% and 1% of the mean. The method has been applied to the analysis of serum from patients undergoing dothiepin therapy.
已开发出一种通过高效液相色谱法分离和测定人血浆或血清中多塞平及其N-去甲基代谢物去甲替林的方法。该方法使用结构相关药物阿米替林作为内标，每种成分的检测限约为10 ng/ml。在浓度为20 ng/ml时，去甲替林和多塞平的测量值分别为平均值的±11%和±6%，在200 ng/ml时为平均值的±3%和1%以内。该方法已应用于接受多塞平治疗患者的血清分析。
