Taikang Tongji (Wuhan) Hospital, China.
Department of Obstetrics, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, China.
Comput Math Methods Med. 2022 Jul 6;2022:3809792. doi: 10.1155/2022/3809792. eCollection 2022.
Telocytes (TCs), a novel interstitial cell type in the reproductive tract, participating in pathophysiology of intrauterine adhesions (IUA). This study further investigates the hypothesis that TCs, a source of Wnt, promote the regeneration and repair of IUA.
RNA sequencing datasets of IUA patient (GSE160633) and mouse intestine mesenchymal cells (GSE94072) in GEO database were analyzed for differentially expressed genes (DEGs), and quantitative real-time PCR (qRT-PCR) measured indicated gene expression in TC-educated endometrial stromal cells (ESCs) and noneducated ESCs and verified the results of data mining from GEO database.
The expression levels of Wnt genes were downregulated in IUA compared to the control and were upregulated in TCs. In particular, the changes of Wnt5a expression level were the most significant (logFC = 4.0314 and adjusted value = 0.0023), and the relative Wnt5a expression level was remarkably higher in TC-educated ESCs than noneducated ESCs verified by qRT-PCR ( = 0.0027).
TCs may enhance the regeneration and repair of IUA through the Wnt signaling pathway.
位于生殖道中的新型间质细胞—— 间质细胞（telocytes，TCs），参与宫腔粘连（intrauterine adhesions，IUA）的病理生理学过程。本研究进一步验证了 TCs 是 Wnt 的来源这一假说，即 TCs 可促进 IUA 的再生和修复。
在 GEO 数据库中分析了 IUA 患者（GSE160633）和小鼠肠间充质细胞（GSE94072）的 RNA 测序数据集，以鉴定差异表达基因（differentially expressed genes，DEGs），并通过实时定量 PCR（quantitative real-time PCR，qRT-PCR）检测 TC 诱导的子宫内膜基质细胞（endometrial stromal cells，ESCs）和未诱导的 ESCs 中特定基因的表达，以验证 GEO 数据库中数据挖掘的结果。
与对照组相比，IUA 中 Wnt 基因的表达水平下调，而在 TCs 中上调。特别是 Wnt5a 表达水平的变化最为显著（logFC = 4.0314，调整 值 = 0.0023），qRT-PCR 验证了 TC 诱导的 ESCs 中 Wnt5a 的相对表达水平明显高于未诱导的 ESCs（ = 0.0027）。
TCs 可能通过 Wnt 信号通路增强 IUA 的再生和修复。
