Wang Yang, Li Xiao, Yuan Juzheng, Wang Xudan, Tao Kaishan, Yan Jin
National and Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Department of Tumor and Immunology in Precision Medical Institute, Western China Science and Technology Innovation Port, Xi'an, China.
Front Bioeng Biotechnol. 2022 Jun 30;10:901534. doi: 10.3389/fbioe.2022.901534. eCollection 2022.
Bionic self-assembly hydrogel derived by peptide as an effective biomedical hemostatic agent has always gained great attention. However, developing hydrogels with eminent-biosecurity, rapidly hemostatic and bactericidal function remains a critical challenge. Hence, we designed an injectable hydrogel with hemostatic and bactericidal function based on Bionic Self-Assembling Peptide (BSAP) in this study. BSAP was formed with two functionalized peptides containing (RADA) motif and possessed the ability to self-assemble into nanofibers. As expected, BSAP could rapidly co-assemble into hydrogel network structure driven by Ca. The hydrogel with a concentration of 5% showed a superior microporous structure and excellent shear thinning characteristics, as well as injectability. Moreover, in the foot trauma model and tail amputation model, the fabricated hydrogel exhibited a lower blood clotting index and dramatically reduced blood clotting time and bleeding volume. Remarkably, the hydrogel reduced inflammatory responses by blocking bacterial infection, promoting wound healing. Finally, the hydrogel is highly hemocompatible and has no cytotoxicity. Overall, this work provides a strategy for developing a high-biosecurity hydrogel with hemostatic and antibacterial properties, which will allow for the clinical application of BSAP.
由肽衍生的仿生自组装水凝胶作为一种有效的生物医学止血剂一直备受关注。然而，开发具有卓越生物安全性、快速止血和杀菌功能的水凝胶仍然是一项严峻挑战。因此，在本研究中，我们基于仿生自组装肽（BSAP）设计了一种具有止血和杀菌功能的可注射水凝胶。BSAP由两个含有（RADA）基序的功能化肽形成，并具有自组装成纳米纤维的能力。正如预期的那样，BSAP在Ca的驱动下能够迅速共组装成水凝胶网络结构。浓度为5%的水凝胶表现出优异的微孔结构、出色的剪切变稀特性以及可注射性。此外，在足部创伤模型和尾部截肢模型中，制备的水凝胶表现出较低的凝血指数，并显著缩短了凝血时间和出血量。值得注意的是，该水凝胶通过阻止细菌感染、促进伤口愈合来减轻炎症反应。最后，该水凝胶具有高度的血液相容性且无细胞毒性。总体而言，这项工作为开发具有止血和抗菌特性的高生物安全性水凝胶提供了一种策略，这将使BSAP能够应用于临床。
