Central Laboratory, Shanghai Tenth People's Hospital, Tongji University, 301# Yanchang Middle Road, Shanghai, 200072, China.
Department of Laboratory Medicine, Shanghai Tenth People's Hospital, Tongji University, 301# Yanchang Middle Road, Shanghai, 200072, China.
Adv Sci (Weinh). 2021 Dec;8(24):e2102545. doi: 10.1002/advs.202102545. Epub 2021 Oct 31.
Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin microbial ecosystem. Here, a living bacterial hydrogel scaffold is reported that accelerates infected wound healing through beneficial bacteria secreting antibacterial substances. Lactobacillus reuteri, a common probiotic, is encapsulated in hydrogel microspheres by emulsion polymerization and further immobilized in a hydrogel network by covalent cross-linking of methacrylate-modified hyaluronic acid. Owing to light-initiated crosslinking, the hydrogel dressing can be generated in situ at the wound site. This hydrogel scaffold not only protects bacteria from immune system attack, but also prevents bacteria from escaping into the local environment, thus avoiding potential threats. Both in vitro and in vivo experiments show that it has excellent ability against harmful bacteria and anti-inflammatory capabilities, promoting infected wound closure and new tissue regeneration. This work may open up new avenues for the application of living bacteria in the clinical management of infected wounds.
受损的皮肤无法阻止有害细菌侵入组织，导致感染的伤口，甚至严重的组织损伤。传统的治疗方法不仅能杀死病原菌，还能抑制有益细菌的生长，从而破坏受损皮肤微生物生态系统的平衡。在这里，我们报道了一种活菌水凝胶支架，它通过有益细菌分泌抗菌物质来加速感染性伤口的愈合。将常见的益生菌罗伊氏乳杆菌通过乳液聚合包封在水凝胶微球中，并通过甲基丙烯酰化透明质酸的共价交联进一步固定在水凝胶网络中。由于光引发交联，水凝胶敷料可以在伤口部位原位生成。这种水凝胶支架不仅可以保护细菌免受免疫系统的攻击，还可以防止细菌逃到局部环境中，从而避免潜在的威胁。体外和体内实验均表明，它对有害细菌具有优异的抗菌能力和抗炎能力，能促进感染性伤口的闭合和新组织的再生。这项工作可能为活菌在感染性伤口的临床治疗中的应用开辟新的途径。
