1. Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran. 2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Biology, Sana Institute of Higher education, Sari, Iran.
BMC Biotechnol. 2021 Dec 7;21(1):68. doi: 10.1186/s12896-021-00727-1.
Antibiotics have been widely used for the treatment of bacterial infections for decades. However, the rapid emergence of antibiotic-resistant bacteria has created many problems with a heavy burden for the medical community. Therefore, the use of nanoparticles as an alternative for antibacterial activity has been explored. In this context, metal nanoparticles have demonstrated broad-spectrum antimicrobial activity. This study investigated the antimicrobial activity of naked cerium oxide nanoparticles dispersed in aqueous solution (CNPs) and surface-stabilized using Pseudomonas aeruginosa as a bacterial model.
Gelatin-polycaprolactone nanofibers containing CNPs (Scaffold@CNPs) were synthesized, and their effect on P. aeruginosa was investigated. The minimum inhibitory and bactericidal concentrations of the nanoparticls were determined in an ATCC reference strain and a clinical isolate strain. To determine whether the exposure to the nanocomposites might change the expression of antibiotic resistance, the expression of the genes shv, kpc, and imp was also investigated. Moreover, the cytotoxicity of the CNPs was assessed on fibroblast using flow cytometry.
Minimum bactericidal concentrations for the ATCC and the clinical isolate of 50 µg/mL and 200 µg/mL were measured, respectively, when the CNPs were used. In the case of the Scaffold@CNPs, the bactericidal effect was 50 µg/mL and 100 µg/mL for the ATCC and clinical isolate, respectively. Interestingly, the exposure to the Scaffold@CNPs significantly decreased the expression of the genes shv, kpc, and imp.
A concentration of CNPs and scaffold@CNPs higher than 50 μg/mL can be used to inhibit the growth of P. aeruginosa. The fact that the scaffold@CNPs significantly reduced the expression of resistance genes, it has the potential to be used for medical applications such as wound dressings.
几十年来，抗生素一直被广泛用于治疗细菌感染。然而，抗生素耐药菌的迅速出现给医学界带来了许多问题。因此，人们探索了将纳米粒子用作抗菌活性的替代物。在这种情况下，金属纳米粒子已经表现出广谱抗菌活性。本研究以铜绿假单胞菌作为细菌模型，研究了分散在水溶液中的裸氧化铈纳米粒子（CNPs）和用铜绿假单胞菌表面稳定的纳米粒子（Scaffold@CNPs）的抗菌活性。
合成了含有 CNPs 的明胶-聚己内酯纳米纤维（Scaffold@CNPs），并研究了它们对铜绿假单胞菌的影响。在 ATCC 参考菌株和临床分离株中确定了纳米粒子的最小抑菌和杀菌浓度。为了确定暴露于纳米复合材料是否会改变抗生素耐药性的表达，还研究了 shv、kpc 和 imp 基因的表达。此外，还使用流式细胞术评估了 CNPs 对成纤维细胞的细胞毒性。
当使用 CNPs 时，分别测量到 ATCC 和临床分离株的最小杀菌浓度为 50μg/mL 和 200μg/mL。对于 Scaffold@CNPs，ATCC 和临床分离株的杀菌作用分别为 50μg/mL 和 100μg/mL。有趣的是，暴露于 Scaffold@CNPs 显著降低了 shv、kpc 和 imp 基因的表达。
CNPs 和 Scaffold@CNPs 的浓度高于 50μg/mL 可用于抑制铜绿假单胞菌的生长。由于 Scaffold@CNPs 显著降低了耐药基因的表达，因此它有可能用于医疗应用，如伤口敷料。
