Hulsebosch C E, Perez-Polo J R, Coggeshall R E
J Comp Neurol. 1987 May 15;259(3):445-51. doi: 10.1002/cne.902590310.
Newborn rats were given subcutaneous injections of antibodies to mouse beta -NGF (ANTI-NGF) daily for 1 month. The number of neurons in T4-T6 dorsal root ganglia (DRG) and the numbers of myelinated and unmyelinated axons in the dorsal roots of the same segments were counted in the ANTI-NGF animals and in normal littermates. The ANTI-NGF rats had 38% fewer neurons in thoracic ganglia but 17% more myelinated and 40% more unmyelinated fibers than their untreated littermates. Dorsal root ganglion cells also have a larger average size in the ANTI-NGF animals, which we interpret as a disproportionate loss of small cells. These data are interpreted as showing that some dorsal root ganglion cells, principally small ones, die when endogenous NGF is inactivated, and that the remaining cells emit more processes than normal. Thus, removal of NGF has what appears to be a paradoxical effect, a reduction in dorsal root ganglion cell numbers but an increase in dorsal root axon numbers. The relation of myelin thickness to fiber diameter is also altered, with small fibers being more thinly myelinated in the ANTI-NGF group. Thus, Schwann cell-neuronal interactions are also affected by inactivation of NGF.
给新生大鼠每日皮下注射抗小鼠β-神经生长因子抗体（抗NGF），持续1个月。在抗NGF组动物和正常同窝仔鼠中，计数T4 - T6背根神经节（DRG）中的神经元数量以及相同节段背根中有髓和无髓轴突的数量。抗NGF大鼠胸神经节中的神经元数量比未处理的同窝仔鼠少38%，但其有髓纤维多17%，无髓纤维多40%。在抗NGF组动物中，背根神经节细胞的平均大小也更大，我们将此解释为小细胞不成比例的减少。这些数据被解释为表明，当内源性NGF失活时，一些背根神经节细胞（主要是小细胞）死亡，而其余细胞发出的突起比正常情况更多。因此，去除NGF产生了一种看似矛盾的效应，即背根神经节细胞数量减少，但背根轴突数量增加。髓鞘厚度与纤维直径的关系也发生了改变，在抗NGF组中小纤维的髓鞘更薄。因此，雪旺细胞与神经元的相互作用也受到NGF失活的影响。
