Bahamdan K A, Khan A R, Tallab T M, Mourad M M
Department of Medicine, College of Medicine, King Saud University, Abha Branch, Kingdom of Saudi Arabia.
Int J Dermatol. 1996 Aug;35(8):558-60. doi: 10.1111/j.1365-4362.1996.tb03654.x.
The clinical diagnosis of cutaneous leishmaniasis is confirmed by demonstrating the organism on a superficial smear or on a biopsy of the lesion. Misdiagnosis in a biopsy specimen may be due to scanty Leishmania organisms that may not be identified in histologic sections.
Punch biopsies of skin lesions, suspected clinically to be cutaneous leishmaniasis, from 29 patients were taken. Touch smears on slides were air-dried, fixed with methyl alcohol, and stained with Giemsa stain. Results of routine histologic examination were then compared with those of touch preparation.
Twenty-one cases were positive for leishmaniasis. In 18 cases, the organisms were seen both in the biopsy and in the touch preparation. In three cases, the organisms were only identified on touch preparation.
A touch preparation improves the sensitivity of the diagnosis of cutaneous leishmaniasis without incurring additional cost to the laboratory.
皮肤利什曼病的临床诊断通过在病变的浅表涂片或活检中发现病原体来确诊。活检标本中的误诊可能是由于利什曼原虫数量稀少，在组织学切片中可能无法识别。
对29例临床疑似皮肤利什曼病的患者的皮肤病变进行钻孔活检。将玻片上的触片空气干燥，用甲醇固定，并用吉姆萨染色。然后将常规组织学检查结果与触片制备结果进行比较。
21例利什曼病呈阳性。18例在活检和触片中均可见病原体。3例仅在触片中发现病原体。
触片制备提高了皮肤利什曼病诊断的敏感性，且不会给实验室带来额外成本。
