Chaudhary Vrantika, Jangra Sumit, Yadav Neelam R
Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar, India.
Front Genet. 2022 Jun 30;13:930113. doi: 10.3389/fgene.2022.930113. eCollection 2022.
Cluster bean popularly known as "guar" is a drought-tolerant, annual legume that has recently emerged as an economically important crop, owing to its high protein and gum content. The guar gum has wide range of applications in food, pharma, and mining industries. India is the leading exporter of various cluster bean-based products all across the globe. Non-coding RNAs (miRNAs) are involved in regulating the expression of the target genes leading to variations in the associated pathways or final protein concentrations. The understanding of miRNAs and their associated targets in cluster bean is yet to be used to its full potential. In the present study, cluster bean EST (Expressed Sequence Tags) database was exploited to identify the miRNA and their predicted targets associated with metabolic and biological processes especially response to diverse biotic and abiotic stimuli using approach. Computational analysis based on cluster bean ESTs led to the identification of 57 miRNAs along with their targets. To the best of our knowledge, this is the first report on identification of miRNAs and their targets using ESTs in cluster bean. The miRNA related to gum metabolism was also identified. Most abundant miRNA families predicted in our study were miR156, miR172, and miR2606. The length of most of the mature miRNAs was found to be 21nt long and the range of minimal folding energy (MFE) was 5.8-177.3 (-kcal/mol) with an average value of 25.4 (-kcal/mol). The identification of cluster bean miRNAs and their targets is predicted to hasten the miRNA discovery, resulting in better knowledge of the role of miRNAs in cluster bean development, physiology, and stress responses.
瓜尔豆俗称“瓜尔”，是一种耐旱的一年生豆科植物，由于其高蛋白和高胶含量，最近已成为一种具有重要经济价值的作物。瓜尔胶在食品、制药和采矿业有广泛应用。印度是全球各种瓜尔豆基产品的主要出口国。非编码RNA（miRNA）参与调节靶基因的表达，导致相关途径或最终蛋白质浓度发生变化。对瓜尔豆中miRNA及其相关靶标的了解尚未得到充分利用。在本研究中，利用瓜尔豆EST（表达序列标签）数据库，采用[具体方法]鉴定与代谢和生物过程相关的miRNA及其预测靶标，特别是对各种生物和非生物刺激的响应。基于瓜尔豆ESTs的计算分析导致鉴定出57个miRNA及其靶标。据我们所知，这是关于利用ESTs鉴定瓜尔豆中miRNA及其靶标的第一份报告。还鉴定了与胶代谢相关的miRNA。在我们的研究中预测的最丰富的miRNA家族是miR156、miR172和miR2606。发现大多数成熟miRNA的长度为21nt，最小折叠能（MFE）范围为5.8 - 177.3（-kcal/mol），平均值为25.4（-kcal/mol）。瓜尔豆miRNA及其靶标的鉴定预计将加速miRNA的发现，从而更好地了解miRNA在瓜尔豆发育、生理和应激反应中的作用。
