Acharya Biswa R, Sandhu Devinder, Dueñas Christian, Ferreira Jorge F S, Grover Kulbhushan K
U.S. Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA 92507, USA.
College of Natural and Agricultural Sciences, University of California Riverside, 900 University Avenue, Riverside, CA 92521, USA.
Plants (Basel). 2022 Jan 22;11(3):291. doi: 10.3390/plants11030291.
Guar is a commercially important legume crop known for guar gum. Guar is tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed to understand molecular mechanisms of salinity tolerance in guar. RNA sequencing (RNA-Seq) was employed to study the leaf and root transcriptomes of salt-tolerant (Matador) and salt-sensitive (PI 340261) guar genotypes under control and salinity. Our analyses identified a total of 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis revealed that the gene expression differences were more pronounced between salinity treatments than between genotypes. Differentially expressed genes associated with stress-signaling pathways, transporters, chromatin remodeling, microRNA biogenesis, and translational machinery play critical roles in guar salinity tolerance. Genes associated with several transporter families that were differentially expressed during salinity included ABC, MFS, GPH, and P-ATPase. Furthermore, genes encoding transcription factors/regulators belonging to several families, including SNF2, CH, bHLH, C3H, and MYB were differentially expressed in response to salinity. This study revealed the importance of various biological pathways during salinity stress and identified several candidate genes that may be used to develop salt-tolerant guar genotypes that might be suitable for cultivation in marginal soils with moderate to high salinity or using degraded water.
瓜尔豆是一种因瓜尔豆胶而具有重要商业价值的豆科作物。瓜尔豆对多种非生物胁迫具有耐受性，但其耐盐性机制尚未完全明确。本研究旨在了解瓜尔豆耐盐性的分子机制。采用RNA测序（RNA-Seq）技术研究了耐盐（Matador）和盐敏感（PI 340261）瓜尔豆基因型在对照和盐胁迫条件下的叶片和根系转录组。我们的分析共鉴定出从5.27亿条clean reads组装得到的296,114个单基因。转录组分析表明，盐处理之间的基因表达差异比基因型之间更为明显。与胁迫信号通路、转运蛋白、染色质重塑、微小RNA生物合成及翻译机制相关的差异表达基因在瓜尔豆耐盐性中起关键作用。在盐胁迫期间差异表达的与几个转运蛋白家族相关的基因包括ABC、MFS、GPH和P-ATPase。此外，编码属于几个家族的转录因子/调控因子的基因，包括SNF2、CH、bHLH、C3H和MYB，在盐胁迫下差异表达。本研究揭示了盐胁迫期间各种生物学途径的重要性，并鉴定出几个候选基因，这些基因可用于培育耐盐瓜尔豆基因型，这些基因型可能适合在中度至高度盐渍化的边缘土壤中种植或使用劣质水进行灌溉。
