Laboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Peru.
Facultad de Ciencias de la Salud, Universidad de Huanuco, Huánuco, Peru.
Front Cell Infect Microbiol. 2022 Jun 30;12:909837. doi: 10.3389/fcimb.2022.909837. eCollection 2022.
Tuberculosis (TB) is one of the most fatal infectious diseases, caused by the aerobic bacteria . It is estimated that one-third of the world's population is infected with the latent (LTB) version of this disease, with only 5-10% of infected individuals developing its active (ATB) form. Pulmonary adenocarcinoma (PA) is the most common and diverse form of primary lung carcinoma. The simultaneous or sequential occurrence of TB and lung cancer in patients has been widely reported and is known to be an issue for diagnosis and surgical treatment. Raising evidence shows that patients cured of TB represent a group at risk for developing PA. In this work, using sRNA-sequencing, we evaluated the expression patterns of circulating small RNAs available in exosomes extracted from blood samples of Peruvian patients affected by latent tuberculosis, active tuberculosis, or pulmonary adenocarcinoma. Differential expression analysis revealed a set of 24 microRNAs perturbed in these diseases, revealing potential biomarker candidates for the Peruvian population. Most of these miRNAs are normally expressed in healthy lung tissue and are potential regulators of different shared and unique KEGG pathways related to cancers, infectious diseases, and immunology.
结核病（TB）是最致命的传染病之一，由需氧细菌引起。据估计，世界上三分之一的人口感染了这种疾病的潜伏（LTB）版本，只有 5-10%的感染者会发展成活动性（ATB）形式。肺腺癌（PA）是原发性肺癌中最常见和最多样化的形式。在患者中同时或先后发生结核病和肺癌已被广泛报道，这是诊断和手术治疗的一个问题。越来越多的证据表明，治愈结核病的患者代表了一组罹患肺腺癌的高危人群。在这项工作中，我们使用 sRNA-seq 技术，评估了从秘鲁潜伏性结核病、活动性结核病或肺腺癌患者血液样本中提取的外泌体中循环小 RNA 的表达模式。差异表达分析显示了一组在这些疾病中受到干扰的 24 个 microRNAs，为秘鲁人群揭示了潜在的生物标志物候选者。这些 miRNA 中的大多数在健康的肺组织中正常表达，是与癌症、传染病和免疫学相关的不同共享和独特 KEGG 途径的潜在调节剂。
