Department of Paediatrics, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Puducherry, India.
Department of Neonatology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Puducherry, India.
Pathog Glob Health. 2020 Jul;114(5):262-270. doi: 10.1080/20477724.2020.1761140. Epub 2020 May 13.
Tuberculosis (TB) is a preventable and curable disease, but increased mortality and morbidity associated with TB is one of the leading causes of deaths worldwide. MicroRNAs (miRNAs) are small, non-coding RNAs known to regulate the host immune response against TB. We investigated the expression profile of candidate circulating miRNAs, which could be used as a blood biomarker for the effective diagnosis of pediatric tuberculosis. A cross-sectional comparative study was conducted, including 30 children with active-TB and 30 healthy controls (HC) in a tertiary care hospital in Puducherry. We used the SYBR green-based miScript qRT-PCR assay to analyze the expression levels of miRNAs in plasma. Further, we used the receiver operating characteristic curve (ROC) to evaluate the diagnostic value of miRNAs. Active-TB included 25 (83.3%) pulmonary TB and 5 (16.7%) extrapulmonary TB cases. We found a significant upregulation of miR-21, miR-29a, miR-31, miR-155, and downregulation of miR-146a in children with active-TB compared to HC. The ROC analysis showed an excellent diagnostic value of miRNAs as follows: miR‑31> miR‑155> miR‑146a with AUC of (95% CI) miRNAs 0.978, 0.953, and 0.903, respectively. Altered circulating miRNA expression levels could be involved in the dysregulation of the host immune response to TB. The ROC analysis indicated that miRNAs miR-31, miR-155 and miR-146a could be effective diagnostic biomarkers for the detection of active-TB in children.
结核病（TB）是一种可预防和可治愈的疾病，但与结核病相关的死亡率和发病率增加是全球主要死亡原因之一。微小 RNA（miRNA）是一种已知可调节宿主对结核病免疫反应的小型非编码 RNA。我们研究了候选循环 miRNA 的表达谱，这些 miRNA 可作为血液生物标志物，用于有效诊断小儿结核病。在浦那的一家三级保健医院进行了一项横断面对比研究，纳入了 30 例活动性结核病患儿和 30 例健康对照（HC）。我们使用 SYBR 绿色基于 miScript qRT-PCR 分析来分析血浆中 miRNA 的表达水平。此外，我们使用接收器操作特征曲线（ROC）来评估 miRNA 的诊断价值。活动性结核病包括 25 例（83.3%）肺结核和 5 例（16.7%）肺外结核病。与 HC 相比，我们发现活动性结核病患儿中 miR-21、miR-29a、miR-31、miR-155 的表达显著上调，miR-146a 的表达下调。ROC 分析显示 miRNA 具有出色的诊断价值，如下所示：miR-31>miR-155>miR-146a，其 AUC（95%CI）分别为 miRNA 0.978、0.953 和 0.903。循环 miRNA 表达水平的改变可能与宿主对结核病免疫反应的失调有关。ROC 分析表明，miR-31、miR-155 和 miR-146a 可能是检测儿童活动性结核病的有效诊断生物标志物。
