Department of Cell and Molecular Biology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India.
Tuberculosis (Edinb). 2019 May;116:37-43. doi: 10.1016/j.tube.2019.04.002. Epub 2019 Apr 17.
MicroRNAs (miRNAs) are a class of noncoding RNA molecules which are involved in various cellular and physiological processes. Previously, studies have identified several miRNAs that are potential diagnostic biomarkers for various infectious diseases including tuberculosis. We have performed small RNA sequencing using the Ion Torrent PGM platform in extra pulmonary tuberculosis (EPTB) subject's serum samples to identify circulating miRNAs during mycobacterium tuberculosis (MTB) infection. Our analysis identified 20 circulating miRNAs upregulated and 5 miRNAs downregulated during MTB infection in patient's serum. In addition, we have identified 6 MTB genome encoded miRNAs upregulated in EPTB patient's serum samples. Taqman based qRT-PCR analysis of host-genome encoded (hsa-miR-146a-5p and hsa-miR-125b-5p) and MTB genome encoded (MTB-miR5) miRNAs showed increased expression in a cohort of 52 healthy, pulmonary tuberculosis (PTB) and extra pulmonary tuberculosis (EPTB) patients serum samples. Our study identified for the first time a panel of host and MTB genome specific differentially expressed circulating miRNAs in serum samples of an Indian patient cohort with tuberculosis infection with a potential as a non-invasive diagnostic biomarker for tuberculosis infection.
微小 RNA（miRNAs）是一类非编码 RNA 分子，参与多种细胞和生理过程。先前的研究已经鉴定出了几种 miRNAs，它们是包括结核病在内的各种传染病的潜在诊断生物标志物。我们使用 Ion Torrent PGM 平台对肺外结核病（EPTB）患者的血清样本进行了小 RNA 测序，以鉴定分枝杆菌结核（MTB）感染期间循环中的 miRNAs。我们的分析鉴定出 20 个在 MTB 感染期间在患者血清中上调的循环 miRNA 和 5 个下调的 miRNA。此外，我们还鉴定出 6 个 MTB 基因组编码的 miRNA 在 EPTB 患者的血清样本中上调。基于 Taqman 的 qRT-PCR 分析宿主基因组编码（hsa-miR-146a-5p 和 hsa-miR-125b-5p）和 MTB 基因组编码（MTB-miR5）miRNAs 的表达，显示在 52 名健康、肺结核（PTB）和肺外肺结核（EPTB）患者的血清样本中表达增加。我们的研究首次在印度结核病感染患者队列的血清样本中鉴定出一组宿主和 MTB 基因组特异性差异表达的循环 miRNAs，它们具有作为结核病感染非侵入性诊断生物标志物的潜力。
