Huang Zhenhua, Liu Lixiang, Huang Shufen, Li Jianbo, Feng Shaozhen, Huang Naya, Ai Zhen, Long Weiqing, Jiang Lanping
The Division of Emergency Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Gynecology, Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China.
Cardiol Res Pract. 2020 Oct 16;2020:6802562. doi: 10.1155/2020/6802562. eCollection 2020.
It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs and its mechanism need further study. Therefore, we investigated whether vitamin D improved the function of EPCs . The peripheral blood mononuclear cells of the participants were isolated from SLE patients and control subjects and cultured to EPCs. After the EPCs were treated with vitamin D (1,25-(OH)D), we evaluated the number, migratory and proliferative activities, and nitric oxide (NO) production of EPCs and detected vascular endothelial function by flow-mediated dilatation (FMD). We found that vitamin D in a dose-dependent manner improved number and migratory and proliferative activities of EPCs from SLE patients. Additionally, vitamin D upregulated NO production from EPCs . A significant correlation between the FMD and plasma NO level was found. There was also a correlation between number, migration, and proliferation of EPCs and NO production. Thus, the present findings indicated that vitamin D improved the function of EPCs from SLE patients via NO secretion.
业已证实，系统性红斑狼疮（SLE）患者体内维生素D水平降低，循环内皮祖细胞（EPCs）功能受损。然而，维生素D对EPCs功能的影响及其机制尚需进一步研究。因此，我们研究了维生素D是否能改善EPCs的功能。从SLE患者和对照受试者中分离出外周血单个核细胞，并培养成EPCs。在用维生素D（1,25 -（OH）D）处理EPCs后，我们评估了EPCs的数量、迁移和增殖活性以及一氧化氮（NO）的产生，并通过血流介导的血管舒张（FMD）检测血管内皮功能。我们发现，维生素D以剂量依赖的方式改善了SLE患者EPCs的数量、迁移和增殖活性。此外，维生素D上调了EPCs产生NO的能力。发现FMD与血浆NO水平之间存在显著相关性。EPCs的数量、迁移和增殖与NO产生之间也存在相关性。因此，目前的研究结果表明，维生素D通过NO分泌改善了SLE患者EPCs的功能。
