七氟醚麻醉对老年狨猴前额叶皮质神经损伤生物标志物的影响
The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets.
作者信息
Cheng Yanyong, Shi Lingling, Mao Haoli, Xue Zhenyu, Liu Siyu, Qiu Zilong, Zhang Lei, Jiang Hong
机构信息
Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
出版信息
Front Aging Neurosci. 2022 Jun 30;14:918640. doi: 10.3389/fnagi.2022.918640. eCollection 2022.
BACKGROUND
Surgery under general anesthesia leads to neural injury, especially in older patients. Sevoflurane anesthesia without surgery for 2 h does not induce neural injury, however, whether prolonger sevoflurane anesthesia without surgery has the same consequence is still unknown.
METHODS
In the present study, aged marmosets were exposed to a clinical concentration of sevoflurane (1.5-2%) for 6 h to access the effects of prolonged sevoflurane anesthesia on the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), Caspase3 activity and myelin formation in the brain.
RESULTS
Sevoflurane anesthesia did not alter the expression of IL-6 (120.1 ± 2.21 vs. 120.8 ± 2.25, = 0.74), TNF-α (189.3 ± 31.35 vs. 218.7 ± 21.47, = 0.25) and Caspase3 (57.35 ± 1.54 vs. 58.67 ± 1.19, = 0.53) in the prefrontal cortex (PFC) of aged marmosets. The amount of MBP expression (60.99 ± 6.21 vs. 58.91 ± 2.71, = 0.77) did not change following sevoflurane exposure.
CONCLUSION
Sevoflurane anesthesia did not increase the levels of IL-6 and TNF-α, activated the the expression of Caspase3, and induced myelination deficits in the PFC of aged marmosets.
背景
全身麻醉下的手术会导致神经损伤，尤其是在老年患者中。然而，七氟醚麻醉2小时但不进行手术不会诱发神经损伤，那么延长非手术状态下的七氟醚麻醉是否会产生相同的结果仍不清楚。
方法
在本研究中，将老年狨猴暴露于临床浓度的七氟醚（1.5 - 2%）6小时，以研究延长七氟醚麻醉对老年狨猴大脑中白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）水平、半胱天冬酶3（Caspase3）活性和髓鞘形成的影响。
结果
七氟醚麻醉并未改变老年狨猴前额叶皮质（PFC）中IL-6（120.1 ± 2.21对120.8 ± 2.25，P = 0.74）、TNF-α（189.3 ± 31.35对218.7 ± 21.47，P = 0.25）和Caspase3（57.35 ± 1.54对58.67 ± 1.19，P = 0.53）的表达。七氟醚暴露后髓鞘碱性蛋白（MBP）表达量（60.99 ± 6.21对58.91 ± 2.71，P = 0.77）未发生变化。
结论
七氟醚麻醉并未增加老年狨猴PFC中IL-6和TNF-α的水平，未激活Caspase3的表达，也未诱导髓鞘形成缺陷。
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