Miao Zhengjie, Jiang Yi, Wang Fangfang, Shi Lingling, Zhou Ren, Niu Yixuan, Zhang Lei
Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Neurosci. 2024 Aug 8;18:1447743. doi: 10.3389/fnins.2024.1447743. eCollection 2024.
The fragile brain includes both the developing brain in childhood and the deteriorating brain in elderly. While the effects of general anesthesia on the myelin sheath of developing brain have been well-documented, limited research has explored its impact on degenerating brain in elderly individuals.
In our study, aged marmosets in control group were only anesthetized with 6-8% sevoflurane and 100% oxygen (2 L/min) for 1-2 min for anesthesia induction. In addition to anesthesia induction, the anesthesia group was exposed to a clinical concentration of sevoflurane (1.5-2%) for 6 h to maintain anesthesia. After anesthesia, scanning electron microscopy (SEM) and artificial intelligence-assisted image analysis were utilized to observe the effects of general anesthesia on the myelin sheath in prefrontal cortex (PFC) of aged marmosets.
Compared with the control group, our findings revealed no evidence that 6 h of sevoflurane general anesthesia altered the thickness of myelin sheath, the diameter of myelinated axons, and the -ratio in prefrontal cortex of aged marmosets.
Clinical concentration of sevoflurane may have no short-term effect on the myelin sheath in prefrontal cortex of aged marmosets.
脆弱的大脑包括儿童时期发育中的大脑和老年人衰退的大脑。虽然全身麻醉对发育中大脑髓鞘的影响已有充分记录，但对老年人衰退大脑影响的研究却很有限。
在我们的研究中，对照组的老年狨猴仅用6 - 8%的七氟醚和100%氧气（2升/分钟）麻醉1 - 2分钟用于诱导麻醉。除诱导麻醉外，麻醉组暴露于临床浓度的七氟醚（1.5 - 2%）6小时以维持麻醉。麻醉后，利用扫描电子显微镜（SEM）和人工智能辅助图像分析观察全身麻醉对老年狨猴前额叶皮质（PFC）髓鞘的影响。
与对照组相比，我们的研究结果表明，没有证据显示6小时的七氟醚全身麻醉会改变老年狨猴前额叶皮质髓鞘的厚度、有髓轴突的直径以及-比率。
临床浓度的七氟醚可能对老年狨猴前额叶皮质的髓鞘没有短期影响。
