Mao Haoli, Zhu Jiao, Cheng Yanyong, Shi Lingling, Chen Xiao, Zhou Ren, Xue Zhenyu, Liu Siyu, Qiu Zilong, Jiang Hong
Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
Front Mol Neurosci. 2022 Jul 6;15:915570. doi: 10.3389/fnmol.2022.915570. eCollection 2022.
In the lipid-rich brain, lipids performed signaling processes associated with the control system of the cell cycle, stress, and inflammatory reactions, as well as maintained brain and cellular homeostasis. The effects of general anesthesia on brain impairment in the elderly were controversial and complex. The study sought to evaluate the effect of lipid metabolism in the brain of aged marmosets and mice under long-term exposure to sevoflurane.
A total of 6 marmosets over 8-year-old and 10 mice aged 18 months were divided into the sevoflurane anesthesia and control groups, respectively. Marmosets in the sevoflurane anesthesia group were exposed to 1.5-2.5% sevoflurane and 100% O for 6 h. Mice anesthetized with sevoflurane were exposed to 3% sevoflurane and 60% O for 6 h. All prefrontal cortex tissues of marmosets and mice were harvested for the analysis of lipidomics.
Compared to the control group, we found that phosphatidylethanolamine (PE) (18:0/22:5), PE (16:0/22:5), PE (18:2/22:5), PE (14:0/22:5), and PE (18:1/22:5) increased in the prefrontal cortex of marmosets in the sevoflurane group, while triglyceride (TAG)56:5-fatty acid (FA) 20:4, TAG58:10-FA22:6, and TAG60:10-FA22:6 decreased. For aged mice, we indicated that lipid components phosphatidic acid (PA) (18:1/20:2) and TAG52:5-FA20:4 in the sevoflurane group increased, but PE (14:0/22:4), diglyceride (DAG) (16:1/18:2), and lysophosphatidylcholine (LPC) (16:1) + AcO decreased. More deeply, sevoflurane anesthesia resulted in the presence of 70 specific lipids in mice and marmosets. The enriched lipid subclasses were mainly monoacylglycerophosphoethanolamines and five other subclasses.
Sevoflurane caused slight changes in lipid metabolism both in the aged brain of marmosets and mice. However, the pathways of lipid metabolism were not affected. The effects of sevoflurane on lipid metabolism in aged brains may differ among species.
在富含脂质的大脑中，脂质执行与细胞周期控制系统、应激和炎症反应相关的信号传导过程，并维持大脑和细胞的稳态。全身麻醉对老年人脑损伤的影响存在争议且复杂。本研究旨在评估长期暴露于七氟醚下老年狨猴和小鼠大脑中脂质代谢的影响。
将6只8岁以上的狨猴和10只18个月大的小鼠分别分为七氟醚麻醉组和对照组。七氟醚麻醉组的狨猴暴露于1.5 - 2.5%的七氟醚和100%的氧气中6小时。用七氟醚麻醉的小鼠暴露于3%的七氟醚和60%的氧气中6小时。收集所有狨猴和小鼠的前额叶皮质组织进行脂质组学分析。
与对照组相比，我们发现七氟醚组狨猴前额叶皮质中磷脂酰乙醇胺（PE）（18:0/22:5）、PE（16:0/22:5）、PE（18:2/22:5）、PE（14:0/22:5）和PE（18:1/22:5）增加，而甘油三酯（TAG）56:5 - 脂肪酸（FA）20:4、TAG58:10 - FA22:6和TAG60:10 - FA22:6减少。对于老年小鼠，我们发现七氟醚组中的脂质成分磷脂酸（PA）（18:1/20:2）和TAG52:5 - FA20:4增加，但PE（14:0/22:4）、甘油二酯（DAG）（16:1/18:2）和溶血磷脂酰胆碱（LPC）（16:1）+ AcO减少。更深入地，七氟醚麻醉导致小鼠和狨猴中出现70种特定脂质。富集的脂质亚类主要是单酰甘油磷酸乙醇胺和其他五个亚类。
七氟醚在老年狨猴和小鼠的大脑中引起了脂质代谢的轻微变化。然而，脂质代谢途径未受影响。七氟醚对老年大脑脂质代谢的影响可能因物种而异。
