Department of Methodology, Medical University of Warsaw, Warsaw, Poland.
Department of Pathology, Professor Witold Orlowski Public Clinical Hospital, Medical Center of Postgraduate Education, Warsaw, Poland.
Pol J Pathol. 2022;73(1):50-59. doi: 10.5114/pjp.2022.117178.
Alterations of PD1/PD-L1 pathway may be associated with an excessive inflammatory response in the intestinal wall in inflammatory bowel diseases (IBD). To evaluate the expression of PD-1 and PD-L1 in 4 compartments of intestinal wall (mucosa, submucosa, muscularis propria and lymphatic follicles), high-resolution immunohistochemically stained slides were obtained from formalin-fixed paraffin-embedded samples of 10 Crohn's disease (CD), 9 ulcerative colitis (UC) and 10 unaffected individuals cases. The levels of expression were quantified using the QuPath software. PD-1 was detected in lymphatic follicles in affected and unaffected tissue samples and in inflammatory infiltration in IBD. There was no difference between groups neither in PD-1 overall expression nor in individual compartments, with the exception of the mucosal expression. It was higher in the mucosa of CD patients comparing to controls, however this difference was marginal (p = 0.0461). PD-L1 was expressed in endothelium and mesenteric nervous plexi, consistently in each group. There were no significant differences in PD-L1 immunoreactivity in context of histologic compartment nor clinical diagnosis. The results suggest that PD-1 and PD-L1 expression in intestinal tissue is heterogeneous in the analysed groups, thus it may be dependent on individual characteristics.
PD1/PD-L1 通路的改变可能与炎症性肠病 (IBD) 肠壁的过度炎症反应有关。为了评估 PD-1 和 PD-L1 在肠壁 4 个部位（黏膜、黏膜下层、固有肌层和淋巴滤泡）的表达，我们从 10 例克罗恩病 (CD)、9 例溃疡性结肠炎 (UC) 和 10 例无病变个体的福尔马林固定石蜡包埋样本中获得了高分辨率免疫组化染色切片。使用 QuPath 软件对表达水平进行定量。PD-1 在受累和未受累组织样本的淋巴滤泡和 IBD 的炎症浸润中均有检测到。各组之间无论是 PD-1 的总体表达还是各部位的表达均无差异，除了黏膜表达。CD 患者的黏膜表达高于对照组，但差异很小（p = 0.0461）。PD-L1 在肠壁内皮细胞和肠系膜神经丛中表达，在每组中均一致表达。在组织学部位和临床诊断方面，PD-L1 免疫反应均无显著差异。研究结果表明，在分析的各组中，肠组织中 PD-1 和 PD-L1 的表达存在异质性，因此可能依赖于个体特征。
