School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Jiangsu Haosen Pharmaceutical Group Co., Ltd, Lianyungang, China.
J Food Biochem. 2022 Oct;46(10):e14316. doi: 10.1111/jfbc.14316. Epub 2022 Jul 18.
Polyphenols are the main active components in Vitis amurensis Rupr. wine, which show good protective effects on the nervous system, but their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. The purpose of this study is to investigate the potential mechanism of the neuroprotective effect of Vitis amurensis Rupr. wine polyphenols on the basis of the specific composition of polyphenols in Vitis amurensis Rupr. wine. In this study, 40 phenolic compounds which include 15 anthocyanins, 10 flavonoids, 10 phenolic acids, 3 proanthocyanidins and 2 resveratrols were identified by UPLC Q-Exactive Orbitrap MS. Furthermore, An UPLC-QqQ/MS method was established to simultaneously determine the phenolic compounds in Vitis amurensis Rupr. Wine, and analyze the content differences of phenolic compounds between Vitis amurensis Rupr. and Vitis vinifera wine. Finally, network pharmacology was employed for the first time to predict the possible pharmacological mechanisms of Vitis amurensis wine polyphenols against nervous damage. Multivariate network analysis indicated that quercetin, (-)-epigallocatechin and various anthocyanins were found as prominent compounds for the treatment of nervous system diseases. Vitis amurensis Rupr. wine polyphenols mainly acted on these key targets, including AKT1, EGFR, ESR1, and SRC, and further regulate the PI3K-AKT and Rap1 signaling pathway for treating nervous system diseases. Our findings suggested that polyphenols in Vitis amurensis Rupr. wine possess neuroprotective effect through multicomponent, multitarget, and multichannel. PRACTICAL APPLICATIONS: Studies have revealed that Vitis amurensis Rupr. has higher levels of phenolic compounds than Vitis vinifera wine. However, due to the few and limited study of Vitis amurensis Rupr., their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. This research uses a holistic network pharmacology strategy to investigate the potential targets and pharmacological mechanisms of Vitis amurensis Rupr. wine polyphenols against nervous damage. To some things up, The finding elucidates the relationships between signaling pathways, targets, and compounds in Vitis amurensis Rupr. wine polyphenols, which may provide guidance and foundations for further application of medicinal food.
多酚是山葡萄（Vitis amurensis Rupr.）葡萄酒中的主要活性成分，对神经系统具有良好的保护作用，但山葡萄葡萄酒中多酚的组成及其神经保护的分子机制尚不清楚。本研究旨在基于山葡萄葡萄酒中多酚的特定组成，探讨山葡萄葡萄酒多酚的神经保护作用的潜在机制。在这项研究中，通过 UPLC Q-Exactive Orbitrap MS 鉴定了 40 种酚类化合物，包括 15 种花色苷、10 种类黄酮、10 种酚酸、3 种原花青素和 2 种白藜芦醇。此外，建立了一种 UPLC-QqQ/MS 方法，同时测定山葡萄葡萄酒中的酚类化合物，并分析山葡萄葡萄酒与酿酒葡萄（Vitis vinifera）葡萄酒中酚类化合物的含量差异。最后，首次采用网络药理学预测山葡萄葡萄酒多酚对神经损伤的可能药理机制。多元网络分析表明，槲皮素、（-）-表儿茶素和各种花色苷被发现是治疗神经系统疾病的突出化合物。山葡萄葡萄酒多酚主要作用于这些关键靶点，包括 AKT1、EGFR、ESR1 和 SRC，并进一步调节 PI3K-AKT 和 Rap1 信号通路，以治疗神经系统疾病。我们的研究结果表明，山葡萄葡萄酒多酚通过多成分、多靶点和多途径发挥神经保护作用。 实际应用：研究表明，山葡萄（Vitis amurensis Rupr.）的酚类化合物含量高于酿酒葡萄（Vitis vinifera）葡萄酒。然而，由于对山葡萄（Vitis amurensis Rupr.）的研究较少且有限，其在山葡萄（Vitis amurensis Rupr.）葡萄酒中的组成及其神经保护的分子机制仍不清楚。本研究采用整体网络药理学策略研究山葡萄（Vitis amurensis Rupr.）葡萄酒多酚对神经损伤的潜在靶点和药理机制。综上所述，该研究阐明了山葡萄（Vitis amurensis Rupr.）葡萄酒多酚在信号通路、靶点和化合物之间的关系，可为进一步应用药食同源品提供指导和依据。
